AMPA Receptor (GluR 4) (D41A11) XP? Rabbit mAb

• 产品编号：CST-8070P      品牌：Cell Signaling Technology       原厂货号：8070P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb 兔单抗识别内源性的总GluR 4蛋白。该单克隆抗体由合成的人类GluR 4蛋白Gln890位点附近肽段免疫动物而制成。AMPA（α-氨基-3-羟基-5-甲基-4-恶唑酸），kainite和NMDA（N-甲基-D-天冬氨酸）受体是离子型谷氨酸门控离子通道的三个主要的家族。AMPA受体（AMPARs）是由四个亚基（GluR1-4）构成，以同源或异源四聚体的形式介导大多数中枢神经系统的兴奋性传递。AMPARs与突触的形成，稳定和可塑性有关（1）。缺乏GluR2的AMPARs可渗透钙，包含GluR2的AMPARs相反（2）。转录后修饰（剪接，RNA编辑核）和翻译后修饰（糖基化，磷酸化）可以导致结果的多样，可以微调AMPARs的动力学性质。AMPARs酶活性的变化与各种疾病有关，包括阿尔茨海默氏症，肌萎缩性侧索硬化症（ALS），中风，癫痫（1）。包含GluR 4的AMPA受体在突触中被发现，GluR 4转运到突触以及细胞表面是由PKA或PKC的Ser842磷酸化所介导的（3）。

1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
3. Gomes, A.R. et al. (2007) Traffic 8, 259-69.

原厂资料：

Specificity / Sensitivity

AMPA Receptor (GluR 4) (D41A11) XP® Rabbit mAb detects endogenous levels of total GluR 4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln890 of human GluR 4 protein.

Background

AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainite- and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the CNS. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). AMPARs that lack GluR 2 are permeable to calcium, in contrast to GluR 2 containing AMPARs (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Activity changes of AMPARs are implicated in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).GluR 4 containing AMPARs are found in synapses and GluR 4 delivery to synapses and cell surface expression is mediated through phosphorylation of Ser842 by PKA or PKC (3).

1. Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
2. Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
3. Gomes, A.R. et al. (2007) Traffic 8, 259-69.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody