SATB1 (D41G7) Rabbit mAb

• 产品编号：CST-8067S      品牌：CST       原厂货号：8067S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SATB1 (D41G7) Rabbit mAb兔单抗能够检测内源性SATB1总蛋白水平。通过合成的与人源SATB1蛋白Pro629位点相应多肽片段去免疫动物从而制备出此单克隆抗体。Special AT-rich binding protein 1 (SATB1)的功能是作为一种宏观基因调控因子和一个特异基因转录因子(1)。SATB1可与promyelocytic leukemia protein (PML)协作去调节宏观染色质结构，通过基质结合区(matrix attachment regions，MARs)将染色质DNA形成的固定到核基质，将组织染色质进入明显袢环状(loop)结构(1-3)。此外，SATB1招募多种染色质重塑蛋白，这些蛋白质有助于特异性基因激活和抑制，包括染色质重塑激酶ACF和ISWI、组蛋白去乙酰酶HDAC1以及组蛋白乙酰转移酶PCAF和p300/CBP (4-6)。由蛋白激酶C使SATB1蛋白Ser185位点的磷酸化能够调节它与HDAC1和PCAF蛋白的相互作用。当非磷酸化的SATB1结合到PCAF上时，磷酸化的 SATB1有倾向性的结合到HDAC1上(6)。由PCAF使SATB1蛋白Lys136位点的乙酰化可减弱它的DNA结合活性，因此可移走基因启动子上的SATB1(6)。SATB1主要表达在胸腺细胞中，并且涉及在T细胞活化期间的基因调节(1)。SATB1也表达在转移性乳腺癌细胞中，并且对于转移性乳腺癌是一个潜在预后标志和治疗靶点(7)。在一个小鼠模型系统中，当在非转移性乳腺癌细胞中SATB1的异常表达可诱发侵入性肿瘤，RNAi介导的SATB1的敲除通过抑制肿瘤生长和转移可扭转肿瘤发生。

1. Galande, S. et al. (2007) Curr Opin Genet Dev 17, 408-14.
2. Cai, S. et al. (2006) Nat Genet 38, 1278-88.
3. Kumar, P.P. et al. (2007) Nat Cell Biol 9, 45-56.
4. Yasui, D. et al. (2002) Nature 419, 641-5.
5. Kumar, P.P. et al. (2005) Mol Cell Biol 25, 1620-33.
6. Pavan Kumar, P. et al. (2006) Mol Cell 22, 231-43.
7. Han, H.J. et al. (2008) Nature 452, 187-93.

原厂资料：

Specificity / Sensitivity

SATB1 (D41G7) Rabbit mAb recognizes endogenous levels of total SATB1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro629 of human SATB1 protein.

Background

Special AT-rich binding protein 1 (SATB1) functions as both a global chromatin organizer and a gene-specific transcription factor (1). SATB1 cooperates with promyelocytic leukemia protein (PML) to regulate global chromatin architecture by organizing chromatin into distinct loops via periodic anchoring of matrix attachment regions (MARs) in DNA to the nuclear matrix (1-3). In addition, SATB1 recruits multiple chromatin-remodeling proteins that contribute to specific gene activation and repression, including the chromatin remodeling enzymes ACF and ISWI, the histone deacetylase HDAC1, and the histone acetyltransferases PCAF and p300/CBP (4-6). Phosphorylation of SATB1 on Ser185 by protein kinase C regulates its interaction with HDAC1 and PCAF. While unphosphorylated SATB1 binds to PCAF, phosphorylated SATB1 preferentially binds to HDAC1 (6). Acetylation of SATB1 on Lys136 by PCAF impairs its DNA binding activity, thereby removing SATB1 from gene promoters (6). SATB1 is expressed predominantly in thymocytes and is involved in gene regulation during T cell activation (1). SATB1 is also expressed in metastatic breast cancer cells and is a potential prognostic marker and therapeutic target for metastatic breast cancer (7). In a mouse model system, RNAi-mediated knockdown of SATB1 reversed tumorigenesis by inhibiting tumor growth and metastasis, while ectopic expression of SATB1 in non-metastatic breast cancer cells produced invasive tumors.

1. Galande, S. et al. (2007) Curr Opin Genet Dev 17, 408-14.
2. Cai, S. et al. (2006) Nat Genet 38, 1278-88.
3. Kumar, P.P. et al. (2007) Nat Cell Biol 9, 45-56.
4. Yasui, D. et al. (2002) Nature 419, 641-5.
5. Kumar, P.P. et al. (2005) Mol Cell Biol 25, 1620-33.
6. Pavan Kumar, P. et al. (2006) Mol Cell 22, 231-43.
7. Han, H.J. et al. (2008) Nature 452, 187-93.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.