Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate)

• 产品编号：CST-8062S      品牌：CST       原厂货号：8062S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate)只能检测内源的在Tyr701位点磷酸化的Stat1α 和 Stat1β蛋白。此抗体不与对应的酪氨酸磷酸化的Stat家族的其它蛋白交叉反应。此单克隆抗体是通过合成人源对应的Stat1 Tyr701位点周围的磷肽段来免疫动物而获得。CST公司生产的此抗体与藻红蛋白偶联，并通过人源细胞的流式细胞仪的测试。正如预期，藻红蛋白偶联的抗体与未偶联的抗体Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167相同，都能够与同物种中的phospho-Stat1蛋白交叉反应。Stat1转录因子可以被一系列配体所激活(1)， 其中对IFN-α和 IFN-γ的应答非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1形成二聚体, 入核后与DNA结合(4)。 Stat1 蛋白是以Stat1α (91 kDa) 和 剪接变体Stat1β (84 kDa)二聚体的形式存在。在很多细胞中，这两种形式都是通过IFN-α激活。但是只有 Stat1α是被 IFN-γ激活。Stat1这种不正常的激活方式存在于很多肿瘤(5)。除了酪氨酸磷酸化，Stat1 也能通过p38有丝分裂激活蛋白激酶依赖(MAPK)依赖的通路在Ser727位点发生磷酸化而被激活，从而对IFN-α和其它的细胞应激产生应答(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

原厂资料：

Specificity / Sensitivity

Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb (PE Conjugate) recognizes endogenous levels of Stat1 only when phosphorylated at Tyr701. The antibody detects phosphorylated Tyr701 of both Stat1α and Stat1β. It does not cross-react with the corresponding phospho-tyrosines of other Stat proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr701 of human Stat1 protein.

Description

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Stat1 (Tyr701) (58D6) Rabbit mAb #9167.

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.