AIM2 Antibody

• 产品编号：CST-8055S      品牌：Cell Signaling Technology       原厂货号：8055S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

AIM2 Antibody能够检测内源AIM2蛋白总体水平。此多克隆抗体是通过合成人源对应的AIM2 Pro250位点周围的肽段来免疫动物而获得。抗体是通过protein A和多肽亲和层析纯化。AIM2 (黑素瘤缺失 2)是干扰素诱导的蛋白，包含一个N末端pyrin域和C-末端的HIN-200域，在天然免疫和肿瘤发展中起作用(1)。AIM2的表达能够抑制细胞的生长和肿瘤的形成(2,3)。此外，AIM2基因在不稳定直肠癌中显现了频繁的突变并与微卫星有关(4)。AIM2在激活caspase-1中发挥了重要的作用, 蛋白酶对前炎症因子 IL-1β 和 IL-18有作用。Caspase-1 的激活被多蛋白复合体“炎性复合体”所调控(5,6)。不同的炎性复合体被描述包含NLRP1/NALP1, NLRP3/NALP3, IPAF 和AIM2。AIM2的HIN-200域对细胞质双链DNA结合有作用,导致了caspase-1的激活(7-9)。炎性复合体也参与到了结合到AIM2 pyrin 域并结合到ASC/TMS1蛋白的CARD域，并因此而与caspase-1直接作用。作为结果, 表明AIM2对多种病原菌具有重要的感知作用 (10-12)。

1. DeYoung, K.L. et al. (1997) Oncogene 15, 453-7.
2. Chen, I.F. et al. (2006) Mol Cancer Ther 5, 1-7.
3. Patsos, G. et al. (2010) Int J Cancer 126, 1838-49.
4. Woerner, S.M. et al. (2007) Genes Chromosomes Cancer 46, 1080-9.
5. Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.
6. Khare, S. et al. (2010) Crit Rev Immunol 30, 463-87.
7. Roberts, T.L. et al. (2009) Science 323, 1057-60.
8. Hornung, V. et al. (2009) Nature 458, 514-8.
9. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13.
10. Jones, J.W. et al. (2010) Proc Natl Acad Sci USA 107, 9771-6.
11. Fernandes-Alnemri, T. et al. (2010) Nat Immunol 11, 385-93.
12. Kim, S. et al. (2010) Eur J Immunol 40, 1545-51.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

AIM2 Antibody recognizes endogenous levels of total AIM2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro250 of human AIM2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

AIM2 (absent in melanoma 2) is an interferon-inducible protein containing an amino-terminal pyrin domain and carboxy-terminal HIN-200 domain that functions in innate immunity and tumor progression (1). Expression of AIM2 can inhibit cell growth and tumor formation (2,3). Furthermore, the gene for AIM2 shows a high frequency of mutations associated with microsatellite unstable colorectal cancers (4). AIM2 has a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. Caspase-1 activation is regulated by multi-protein complexes referred to as “inflammasomes” (5,6). Distinct inflammasome complexes have been described containing NLRP1/NALP1, NLRP3/NALP3, IPAF, and AIM2. The HIN-200 domain of AIM2 is responsible for binding to cytoplasmic double stranded DNA, resulting in caspase-1 activation. (7-9). This inflammasome complex also involves binding of the pyrin domain of AIM2 to the CARD-domain protein ASC/TMS1, which then interacts directly with caspase-1. As a result, AIM2 has been demonstrated to be an important sensor for a number of different pathogens (10-12).

1. DeYoung, K.L. et al. (1997) Oncogene 15, 453-7.
2. Chen, I.F. et al. (2006) Mol Cancer Ther 5, 1-7.
3. Patsos, G. et al. (2010) Int J Cancer 126, 1838-49.
4. Woerner, S.M. et al. (2007) Genes Chromosomes Cancer 46, 1080-9.
5. Schroder, K. and Tschopp, J. (2010) Cell 140, 821-32.
6. Khare, S. et al. (2010) Crit Rev Immunol 30, 463-87.
7. Roberts, T.L. et al. (2009) Science 323, 1057-60.
8. Hornung, V. et al. (2009) Nature 458, 514-8.
9. Fernandes-Alnemri, T. et al. (2009) Nature 458, 509-13.
10. Jones, J.W. et al. (2010) Proc Natl Acad Sci USA 107, 9771-6.
11. Fernandes-Alnemri, T. et al. (2010) Nat Immunol 11, 385-93.
12. Kim, S. et al. (2010) Eur J Immunol 40, 1545-51.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.