DAZL Antibody

• 产品编号：CST-8042S      品牌：CST       原厂货号：8042S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

DAZL抗体识别内源性的总DAZL蛋白。合成与人源DAZL蛋白邻近羧基末端的氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。人DAZ（在无精子症中缺失）基因家族包含至少三个编码RNA结合蛋白的成员，它们包含有通用的RNA识别基序（1）。DAZ的常染色体同源基因，DAZL(DAZ-like)，在生殖细胞中特异表达，并在胚胎形成和成体配子发生过程中对生殖细胞有关键的作用（2,3）。DAZL可以通过直接招募poly(A)—结合蛋白（PABPs）而在生殖细胞发育过程中激活沉默的mRNA（2）。Y染色体DAZ基因及其附近的缺失是人类不孕不育症的最常见原因（4，5）。

1. Yen, P.H. (2004) Int J Androl 27, 125-9.
2. Collier, B. et al. (2005) EMBO J 24, 2656-66.
3. Brook, M. et al. (2009) Reproduction 137, 595-617.
4. Reijo, R. et al. (1995) Nat Genet 10, 383-93.
5. Cooke, H.J. and Elliott, D.J. (1997) Trends Genet 13, 87-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

DAZL Antibody recognizes endogenous levels of total DAZL protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human DAZL protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The human DAZ (Deleted in AZoospermia) gene family contains at least three members that encode RNA-binding proteins with a common RNA-recognition motif (1). An autosomal homolog of DAZ, DAZL (DAZ-like), is specifically expressed in germ cells and has been found to be essential for the specification of the germ cell lineage during embryogenesis and during gametogenesis adults of both sexes (2,3). DAZL may function by directly recruiting poly(A)-binding proteins (PABPs) in order to activate silent mRNAs during germ cell development (2). Deletions encompassing the Y chromosomal DAZ genes are the most common molecularly defined cause of infertility in humans (4,5).

1. Yen, P.H. (2004) Int J Androl 27, 125-9.
2. Collier, B. et al. (2005) EMBO J 24, 2656-66.
3. Brook, M. et al. (2009) Reproduction 137, 595-617.
4. Reijo, R. et al. (1995) Nat Genet 10, 383-93.
5. Cooke, H.J. and Elliott, D.J. (1997) Trends Genet 13, 87-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.