RBBP5 Antibody

• 产品编号：CST-8037S      品牌：Cell Signaling Technology       原厂货号：8037S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

RBBP5 Antibody能够检测内源性RBBP5总蛋白水平。通过合成的与人源RBBP5蛋白Val407位点周围相应多肽片段去免疫动物从而制备出多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Set1组蛋白甲基转移酶被首先鉴定在酵母中，并且是Set1/COMPASS组蛋白甲基转移酶复合物中的一部分，这能使histone H3蛋白Lys4位点甲基化，以及其功能作为转录辅助激活因子(1)。当酵母含有仅一个已知的Set1蛋白，哺乳动物包含六个Set1相关蛋白：SET1A、SET1B、MLL1、MLL2、MLL3和MLL4，这些蛋白都能装配到COMPASS样复合物，并且可以使histone H3蛋白Lys4位点甲基化(2,3)。这些Set1相关蛋白每一个都被发现在明显的蛋白质复合物中，这些都含有共同亚单位WDR5、RBBP5、ASH2L、CXXC1和DPY30，而这些亚单位对于正确复合物装配和组蛋白甲基转移酶活性的调节是需要的(2-6)。MLL1和MLL2复合物包含额外的蛋白质亚单位menin (6)。像酵母Set1一样，所有六种Set1相关哺乳动物蛋白可以使histone H3蛋白Lys4位点甲基化(2-6)。MLL易位被发现在大量的血液恶性肿瘤中，这认为Set1/COMPASS组蛋白甲基转移酶复合物在白血病生成中起到关键作用 (6)。

1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Bovine, Pig, Horse

Specificity / Sensitivity

RBBP5 Antibody recognizes endogenous levels of total RBBP5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val407 of human RBBP5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The Set1 histone methyltransferase protein was first identified in yeast as part of the Set1/COMPASS histone methyltransferase complex, which methylates histone H3 at Lys4 and functions as a transcriptional co-activator (1). While yeast contain only one known Set1 protein, mammals contain six Set1-related proteins: SET1A, SET1B, MLL1, MLL2, MLL3, and MLL4, all of which assemble into COMPASS-like complexes and methylate histone H3 at Lys4 (2,3). These Set1-related proteins are each found in distinct protein complexes, all of which share the common subunits WDR5, RBBP5, ASH2L, CXXC1 and DPY30, which are required for proper complex assembly and modulation of histone methyltransferase activity (2-6). MLL1 and MLL2 complexes contain the additional protein subunit, menin (6). Like yeast Set1, all six Set1-related mammalian proteins methylate histone H3 at Lys4 (2-6). MLL translocations are found in a large number of hematological malignancies, suggesting that Set1/COMPASS histone methyltransferase complexes play a critical role in leukemogenesis (6).

1. Miller, T. et al. (2001) Proc Natl Acad Sci USA 98, 12902-7.
2. Shilatifard, A. (2008) Curr Opin Cell Biol 20, 341-8.
3. Tenney, K. and Shilatifard, A. (2005) J Cell Biochem 95, 429-36.
4. Lee, J.H. and Skalnik, D.G. (2005) J Biol Chem 280, 41725-31.
5. Lee, J.H. et al. (2007) J Biol Chem 282, 13419-28.
6. Hughes, C.M. et al. (2004) Mol Cell 13, 587-97.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.