GGA3 (D66F1) Rabbit mAb

• 产品编号：CST-8027S      品牌：CST       原厂货号：8027S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

GGA3 (D66F1)Rabbit mAb 兔单抗识别内源性的总GGA3蛋白。此抗体同时还能识别24-32kDa未知蛋白的背景条带。该单克隆抗体由合成的人类GGA3蛋白Leu523位点附近肽段免疫动物而制成。GGA3是包括GGA1和GGA2的GGA蛋白家族成员之一。这些蛋白质包括四个不同的部分：VHS结构域，结合Di-亮氨酸排序信号DXXLL; GAT结构域结合ARF-GTP; 铰链区招募clathrin; GAE结构域具有与γ-adaptin相似的序列，可以招募很多蛋白质。Arf1-GTP酶招募GGA3到高尔基体反面网络。GGAS分拣酸水解酶到溶酶体中，参与从高尔基复合体到内吞体运送含有DXXLL信号的蛋白质（1）。在细胞凋亡或脑缺血时，GGA3被caspase-3在Asp313位置裂解，减少GGA3水平和溶酶体降解β-分泌酶（BACE）的水平。BACE的活性增加和数量增多在β-淀粉样蛋白（Aβ）的形成中发挥作用，在阿尔茨海默氏病中，BACE的升高和Aβ的积累相一致（2）。

1. Bonifacino, J.S. (2004) Nat Rev Mol Cell Biol 5, 23-32.
2. Tesco, G. et al. (2007) Neuron 54, 721-37.

原厂资料：

Specificity / Sensitivity

GGA3 (D66F1) Rabbit mAb recognizes endogenous levels of total GGA3 protein. This antibody may also recognize cross-reacting bands of unknown origin between 25 and 32 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu523 of human GGA3 protein.

Background

GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2).

1. Bonifacino, J.S. (2004) Nat Rev Mol Cell Biol 5, 23-32.
2. Tesco, G. et al. (2007) Neuron 54, 721-37.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.