Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-8024S      品牌：CST       原厂货号：8024S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor488 Conjugate）识别内源性的总Neurofilament-L蛋白。该单克隆抗体由合成的人类Neurofilament-L蛋白Glu450位点附近肽段免疫动物而制成。CST的此抗体与Alexa Fluor488 荧光染料耦联，已通过在人细胞上进行免疫荧光分析的内部测试。该抗体与未偶联的 Neurofilament-L (C28E10) Rabbit mAb 兔单抗#2837 具有相同的种属交叉反应性。细胞骨架由三种胞内纤维构成：微丝，中间纤维和微管。Neurofilament是神经元中发现的主要的中间纤维，是组成轻 (NFL)、中 (NFM)、重 (NFH)三种纤维的亚基。与其它中间纤维蛋白的结构类似，neurofilament也具有球状的氨基端头，中央α-螺旋杆结构域和一个羧基端尾。它的前体纤维由一种异型四聚体单位（NFL-NFM和NFL-NFH）构成，八条这样的前体纤维组成一个典型的10nm的中间纤维。neurofilament在轴突径向生长和确定轴突口径中起作用，微管参与轴突伸长。PKA磷酸化NFL和NFM的头端结构域，抑制neurofilament聚集（3,4）。Neurofilament的聚集在许多人类神经系统疾病中被发现，包括帕金森病（与Lewy小体中α-synuclein伴随），阿尔茨海默氏病，腓骨肌萎缩症，肌萎缩性侧索硬化症（ALS）（1）。

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total Neurofilament-L protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Neurofilament-L (C28E10) Rabbit mAb #2837.

Background

The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Neurofilament accumulations are found in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).

1. Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
2. Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
3. Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
4. Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Protect from light. Do not
freeze.