Phospho-Catenin δ-1 (Ser320) Antibody

• 产品编号：CST-8016S      品牌：CST       原厂货号：8016S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Catenin δ-1 (Ser320) Antibody检测仅在Ser320位点磷酸化的内源性catenin δ-1蛋白。通过人工合成人源catenin δ-1蛋白Ser320位点周围相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。 Catenin δ-1 (p120 catenin)蛋白有一个氨基末端卷曲螺旋结构域，接着是一个调节结构域，其含有许多磷酸化位点和一个十个连接的42氨基酸重复序列的中央 Armadillo重复区域。该蛋白羧基末端含有不知道的功能(1)。Catenin δ-1 蛋白在调节细胞与细胞的粘附实现关键角色，因为它调节E-cadherin蛋白在细胞表面周转从而确定可用于细胞与细胞粘附的E-cadherin蛋白水平(2) 。Catenin δ-1蛋白在钙粘蛋白介导的粘附有正性和负性的影响(3)。 Actin 动力学也通过catenin δ-1蛋白调节，其能RhoA、Rac和cdc42蛋白(1)。类似于β-catenin蛋白，catenin δ-1蛋白定位于细胞核，但是这个定位的功能仍不清楚。许多研究认为catenin δ-1蛋白在不同类型的肿瘤细胞中不相关被表达或缺乏，认为catenin δ-1蛋白可能作为肿瘤抑制因子的功能(4)。Cell Signaling Technology (CST)公司使用PhosphoScan®, a CST™ LC-MS/MS平台发现磷酸化位点，catenin δ-1蛋白在Ser320位点磷酸化被鉴定 (5)。

1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
5. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Phospho-Catenin δ-1 (Ser320) Antibody recognizes endogenous levels of catenin δ-1 protein only when phosphorylated at Ser320. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser320 of human catenin δ-1 protein. Antibodies are purified by protein A and peptide affinity chromatography. 

Background

Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function (1). Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion (2). Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion (3). Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins (1). Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor (4). 

Phosphorylation at Ser320 on catenin δ-1 was identified at Cell Signaling Technology (CST) using PhosphoScan®, a CST™ LC-MS/MS platform for phosphorylation site discovery (5). 

1. Reynolds, A.B. and Roczniak-Ferguson, A. (2004) Oncogene 23, 7947-7956.
2. Davis, M. A. et al. (2003) J. Cell Biol. 163, 525-534.
3. Thoreson, M.A. and Reynolds, A.B. (2002) Differentiation 70, 583-589.
4. Anastasiadis, P.Z. and Reynolds, A.B. (2000) J. Cell Sci. 113, 1319-1334.
5. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

注意事项：

Storage: Supplied in 10 mM HEPES (pH 7.5), 150 mM NaCl,
100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot
the antibody.