Mono-Methyl Arginine [mme-R] MultiMab? Rabbit mAb mix

• 产品编号：CST-8015S      品牌：CST       原厂货号：8015S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Mono-Methyl Arginine (Me-R4-100) Rabbit mAb识别内源性的单甲基精氨酸蛋白。此单甲基精氨酸基序抗体没有序列偏好。此抗体不与精氨酸二甲基化或未甲基化的蛋白有交叉反应。该单克隆抗体用合成的被简并氨基酸包围的单甲基精氨酸基序的肽段免疫动物制备。该抗体是两个兔单克隆群的混合，以便可以覆盖更大的反应范围。精氨酸甲基化是常见的转录后修饰，在核和细胞质蛋白中均发现。精氨酸甲基化蛋白涉及许多细胞过程，包括转录后调节、信号转导、RNA代谢和DNA损伤修复（1-3）。精氨酸甲基化由精氨酸N甲基转移酶（PRMT）家族催化甲基从S-腺苷甲硫氨酸（AdoMet）转移到精氨酸胍氮上而形成（4）。精氨酸甲基化有三种形式：不对称二甲基化（aDMA, omega-NG,NG-二甲基精氨酸），两个甲基基团在精氨酸胍基氮原子的一端；对称二甲基化（sDMA, omega-NG, N'G-二甲基精氨酸），每个甲基基团在精氨酸胍基氮原子的一端；单甲基化（MMA, omega-NG-二甲基精氨酸），只有一个甲基集团在精氨酸胍基氮原子上。每种修饰都有其潜在的不同功能。尽管所有PRMT蛋白都催化MMA的形成，I型PRMT(PRMT1, 3, 4, 6)可以添加一个额外的甲基基团从而产生aDMA，II型PRMT(PRMT5和7)产生sDMA。甲基化精氨酸残基经常在富含甘氨酸-精氨酸（GAR）蛋白结构域中出现，如RGG, RG, RXR重复序列（5）。但是PRMT4/CARM1和PRMT5可以甲基化含脯氨酸-甘氨酸-蛋氨酸（PGM）基序的精氨酸（6）。

1. Bedford, M.T. and Richard, S. (2005) Mol Cell 18, 263-72.
2. Pahlich, S. et al. (2006) Biochim Biophys Acta 1764, 1890-903.
3. Bedford, M.T. and Clarke, S.G. (2009) Mol Cell 33, 1-13.
4. McBride, A.E. and Silver, P.A. (2001) Cell 106, 5-8.
5. Gary, J.D. and Clarke, S. (1998) Prog Nucleic Acid Res Mol Biol 61, 65-131.
6. Cheng, D. et al. (2007) Mol Cell 25, 71-83.
7. Dhar, S. et al. (2013) Sci Rep 3, 1311.

原厂资料：

Specificity / Sensitivity

Mono-Methyl Arginine (Me-R4-100) Rabbit mAb recognizes endogenous levels of mono-methyl arginine protein. This is a general mono-methyl arginine motif antibody without sequence preferences. It does not cross-react with di-methyl arginine or unmethylated arginine.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide library containing mono-methyl arginine surrounded by degenerate amino acids. The antibody is formulated from four rabbit monoclones in order to cover a broad range of reactivity.

Background

Arginine methylation is a prevalent post-translational modification found on both nuclear and cytoplasmic proteins. Arginine methylated proteins are involved in many different cellular processes, including transcriptional regulation, signal transduction, RNA metabolism, and DNA damage repair (1-3). Arginine methylation is carried out by the arginine N-methyltransferase (PRMT) family of enzymes that catalyze the transfer of a methyl group from S-adenosylmethionine (AdoMet) to a guanidine nitrogen of arginine (4). There are three different types of arginine methylation: asymmetric dimethylarginine (aDMA, omega-NG,NG-dimethylarginine), where two methyl groups are placed on one of the terminal nitrogen atoms of the guanidine group of arginine; symmetric dimethylarginine (sDMA, omega-NG,N’G-dimethylarginine), where one methyl group is placed on each of the two terminal guanidine nitrogens of arginine; and monomethylarginine (MMA, omega-NG-dimethylarginine), where a single methyl group is placed on one of the terminal nitrogen atoms of arginine. Each of these modifications has potentially different functional consequences. Though all PRMT proteins catalyze the formation of MMA, Type I PRMTs (PRMT1, 3, 4, and 6) add an additional methyl group to produce aDMA, while Type II PRMTs (PRMT5 and 7) produce sDMA. Methylated arginine residues often reside in glycine-arginine rich (GAR) protein domains, such as RGG, RG, and RXR repeats (5). However, PRMT4/CARM1 and PRMT5 methylate arginine residues within proline-glycine-methionine rich (PGM) motifs (6).

1. Bedford, M.T. and Richard, S. (2005) Mol Cell 18, 263-72.
2. Pahlich, S. et al. (2006) Biochim Biophys Acta 1764, 1890-903.
3. Bedford, M.T. and Clarke, S.G. (2009) Mol Cell 33, 1-13.
4. McBride, A.E. and Silver, P.A. (2001) Cell 106, 5-8.
5. Gary, J.D. and Clarke, S. (1998) Prog Nucleic Acid Res Mol Biol 61, 65-131.
6. Cheng, D. et al. (2007) Mol Cell 25, 71-83.
7. Dhar, S. et al. (2013) Sci Rep 3, 1311.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.