Human His6Thymic Stromal Lymphopoietin (hHis6TSLP)

• 产品编号：CST-8012SF      品牌：CST       原厂货号：8012SF
• 产品分类：蛋白质检测与分析 > 蛋白质，多肽和酶 > 细胞因子和生长因子 > 其它细胞因子和生长因子
• 应用分类：

描述：

CST公司生产的人重组蛋白_His6TSLP (h_His6TSLP) Tyr29-Gln159 (Accession #NP_149024)是从人的293细胞表达而来。重组的h_His6TSLP包含两个点突变(R127A和R130S)，以避免弗林蛋白酶的剪切。重组的TSLP蛋白在N-末端有His6标签，蛋白分子量据推算为17,352Da。DTT-还原的蛋白和未还原的蛋白作为31kDa的蛋白而迁移。在SDS-PAGE中具稍低的迁移率是由于糖基化。重组h_His6TSLP蛋白的N-末端序列通过测序得到。6 μg 还原 (+) 和 非还原的(-) 重组h_His6TSLP通过SDS-PAGE检测，纯度大于98%，所有批次的纯度均高于98%。h_His6TSLP的生物活性是通过诱导PBMC产生TARC 能力的实验来确定的。每个批次的ED50在50-200 pg/ml之间。

有载体: 每微克h_His6TSLP的蛋白溶解在包含 20 μg BSA的PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

无载体：每微克h_His6TSLP的蛋白溶解在PBS（pH 7.2）溶液中，并通过 0.22 μm 滤膜冻干。

TSLP是IL-7样细胞因子，它在促进TH2类型的免疫应答中发挥主要作用(1)。皮肤、肺和肠道中的上皮细胞是TSLP最主要的生产者(1)。一系列的免疫细胞类型，包括树突状细胞、淋巴细胞、肥大细胞、噬碱细胞和其它细胞，都对TSLP有响应(1)。与其能够促进TH2类型免疫应答作用一致，TSLP是抗寄生虫免疫应答的重要成分(1)。另外, TSLP也能通过TH2型炎症促进癌症发展(2-4)。TSLP通过IL-7Rα/TSLPR异源二聚体的信号而激活Jak1/2和 Stat5信号通路(5,6)。

原厂资料：

Source / Purification

Recombinant human His6TSLP (hHis6TSLP) Tyr29-Gln159 (Accession #NP_149024) was expressed in human 293 cells at Cell Signaling Technology. Recombinant hHis6TSLP contains 2 point mutations to eliminate potential furin cleavage sites (R127A and R130S).

Molecular Characterization

Recombinant human N-terminally His6-tagged TSLP has a calculated MW of 17,352. DTT-reduced and non-reduced protein migrate as 31 kDa polypeptides. Lower mobility in SDS-PAGE is due to glycosylation. The expected amino terminus of recombinant hHis6TSLP was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis6TSLP. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hHis6TSLP was determined by its ability to induce TARC production by PBMC. The ED50 of each lot is between 50-200 pg/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hHis6TSLP.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis6TSLP. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

TSLP is an IL-7-like cytokine that plays a key role in promoting TH2–type immune responses (1). Epithelial cells in the skin, lung, and gut are the predominant producers of TSLP (1). A number of immune cell types are responsive to TSLP including dendritic cells, lymphocytes, mast cells, and basophils, among others (1). Consistent with its role as a promoter of TH2-type immune responses, TSLP is a crucial component of the anti-helminth immune response (1). Moreover, TSLP may promote tumor progression by inducing TH2-type inflammation (2-4). TSLP signals through IL-7Rα/TSLPR heterodimers, activating Jak1/2 and Stat5 pathways (5,6).

注意事项：

Storage: Stable in lyophilized state at 4ºC for 1 year after
receipt. Sterile stock solutions reconstituted with carrier protein
are stable at 4ºC for 2 months and at -20ºC for 6 months. Avoid
repeated freeze-thaw cycles