HELLS Antibody

• 产品编号：CST-7998S      品牌：CST       原厂货号：7998S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HELLS Antibody能够检测内源性HELLS总蛋白水平。HELLS Antibody预测可检测这种蛋白的多种亚型。通过合成的与人源HELLS蛋白Lys520位点周围相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。HELLS或LSH1是一个淋巴特异性解旋酶，它被认为涉及细胞内增殖和起发白血病(1,2)。它被认为是一个染色质重塑蛋白，并且对于DNMT1介导甲基化维护和DNMT3A/DNMT3B介导重新甲基化是需要的。HELLS在甲基化维护中的作用被认为是最大程度限制DNA重复序列，包括主要的和次要的卫星序列，而不是单一复制基因(3,4)；最近研究认为在非重复序列中基因组范围内半胱氨酸甲基化的功能(5)。重新甲基化的维持是与特异性基因的沉默有关，一些认为是涉及多潜能和谱系提交(6,7)。

1. Lee, D.W. et al. (2000) Cancer Res 60, 3612-22.
2. Fan, T. et al. Epigenetics 3, 134-42.
3. Dennis, K. et al. (2001) Genes Dev 15, 2940-4.
4. Muegge, K. (2005) Biochem Cell Biol 83, 548-54.
5. Tao, Y. et al. (2011) Proc Natl Acad Sci USA 108, 5626-31.
6. Xi, S. et al. (2009) Stem Cells 27, 2691-702.
7. Xi, S. et al. (2007) Proc Natl Acad Sci USA 104, 14366-71.

原厂资料：

Specificity / Sensitivity

HELLS Antibody recognizes endogenous levels of total HELLS protein. HELLS Antibody is predicted to detect multiple isoforms of this protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys520 of human HELLS protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

HELLS, or LSH1, is a lymphoid-specific helicase thought to be involved in cellular proliferation and leukemogenesis (1,2). It is believed to be a chromatin remodeler and is required for DNMT1-mediated methylation maintenance and DNMT3A/DNMT3B-mediated de novo methylation. The role of HELLS in methylation maintenance was thought to be largely confined to repetitive DNA sequences, including major and minor satellite sequences, rather than single copy genes (3,4); recent evidence suggests a role in genome-wide cysteine methylation at non-repeat sequences (5). De novo methylation maintenance is associated with silencing of specific genes, some known to be involved in pluripotency and lineage commitment (6,7).

1. Lee, D.W. et al. (2000) Cancer Res 60, 3612-22.
2. Fan, T. et al. Epigenetics 3, 134-42.
3. Dennis, K. et al. (2001) Genes Dev 15, 2940-4.
4. Muegge, K. (2005) Biochem Cell Biol 83, 548-54.
5. Tao, Y. et al. (2011) Proc Natl Acad Sci USA 108, 5626-31.
6. Xi, S. et al. (2009) Stem Cells 27, 2691-702.
7. Xi, S. et al. (2007) Proc Natl Acad Sci USA 104, 14366-71.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.