PathScan? Total Acetyl-CoA Carboxylase Sandwich ELISA Kit

• 产品编号：CST-7996C      品牌：CST       原厂货号：7996C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

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描述：

PathScan® Total Acetyl-CoA Carboxylase Sandwich ELISA Kit #7996用于检测内源性乙酰辅酶A合成酶(ACC)总蛋白水平(见图1)。试剂盒敏感性见图2。PathScan® Total Acetyl-CoA Carboxylase (ACC) Sandwich ELISA Kit是一种固相三明治酶联免疫试验(ELISA)试剂盒，用于检测内源性ACC。首先，小鼠ACC抗体涂在微孔中表面，然后与细胞裂解液孵育，裂解液中的ACC蛋白(磷酸化的和非磷酸化的)被捕获到涂层抗体上。将微孔洗脱后，加入兔ACC检测抗体，用于检测被捕获的ACC蛋白。然后加入抗兔IgG抗体，即辣根过氧化物酶耦联抗体( HRP-linked Antibody #7074)，用于检测已经结合的检测抗体。最后加入辣根过氧化物酶的底物，四甲基联苯胺(TMP)，加入底物后发生显色反应。ACC的数量与显色的强度成正比，因此可以借此对ACC定量。乙酰辅酶A合成酶(ACC)用于催化脂肪酸合成途径中的关键步骤。分子量为265 kDa的ACCα，是肝脏，脂肪细胞和乳腺中ACC的主要形式，而在骨胳肌和心脏中，其主要形式为280 kDa的ACCβ(1)。ACC可以在Ser79被AMPK磷酸化，或者在Ser1200被PKA磷酸化，这些位点的磷酸化可以抑制ACC的酶活性(2)。ACC也是减肥药物的一个潜在靶点(3,4)。

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

原厂资料：

Specificity / Sensitivity

PathScan® Total Acetyl-CoA Carboxylase Sandwich ELISA Kit #7996 detects endogenous levels of total ACC protein (see Figure 1). The kit sensitivity is shown in Figure 2.

Description

The PathScan® Total Acetyl-CoA Carboxylase (ACC) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of ACC. An ACC mouse antibody has been coated onto the microwells. After incubation with cell lysates, ACC (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, an ACC rabbit detection antibody is added to detect captured ACC protein. Anti-rabbit IgG, HRP-linked Antibody #7074 is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total ACC.

Background

Acetyl-CoA carboxylase (ACC) catalyzes the pivotal step of the fatty acid synthesis pathway. The 265 kDa ACCα (ACC1) is the predominant isoform found in liver, adipocytes, and mammary gland, while the 280 kDa ACCβ (ACC2) is the major isoform in skeletal muscle and heart (1). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (2). ACC is a potential target of anti-obesity drugs (3,4).

1. Castle, J.C. et al. (2009) PLoS One 4, e4369.
2. Kreuz, S. et al. (2009) Diabetes Metab Res Rev 25, 577-86.
3. Ha, J. et al. (1994) J Biol Chem 269, 22162-8.
4. Abu-Elheiga, L. et al. (2001) Science 291, 2613-6.
5. Levert, K.L. et al. (2002) J Biol Chem 277, 16347-50.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 1X Cell Lysis Buffer, which is stored at –20°C (packaged separately).