PathScan? Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair

• 产品编号：CST-7993S      品牌：Cell Signaling Technology       原厂货号：7993S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体对的特异性和敏感度请参考对应的PathScan® Sandwich ELISA Kit。注意: 此抗体对能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白。CST的PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair是作为PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941更为经济的替代而提供的。捕获和检测抗体和HRP连接的二抗分别是作为100X 存储液和1000X存储液而提供。此抗体对为4 x 96孔板的ELISAs提供了足够的试剂。phospho-Lck (Tyr505)鼠捕获抗体在PBS中加入到96孔板中过夜。封闭后, 加入细胞裂解液然后加入总Lck 鼠检测抗体和抗鼠IgG, HRP连接抗体。 HRP 的底物TMB加入进行显色反应。对反应的颜色，吸收值的增大按比例换算成phospho-Lck (Tyr505)蛋白的量。Lck 属于Src样无受体酪氨酸激酶家族，具有典型的Src家族激酶的结构: 一个独特的N-末端域(Src同源4结构域, SH4) 然后是一个SH3 结构域, 一个SH2 结构域, 激酶结构域(SH1)和C-末端负调节结构域(1)。Lck的活性受到SH2和SH3区域的相互作用和在活性环内酪氨酸磷酸化的状态的共同调控(2,3)。受到刺激后，Lck蛋白被招募到T细胞受体(TCR)复合体上并激活下游酪氨酸激酶起始的T细胞信号通路(4)。Lck被发现也参与到线粒体凋亡信号通路的调控中，可能也对一些抗癌药物诱导凋亡作出反应(5,6)。

1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27.
3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
5. Belka, C. et al. (2003) Oncogene 22, 176-85.
6. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

Cell Signaling Technology's PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Lck (Tyr505) Sandwich ELISA Kit #7941. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The phospho-Lck (Tyr505) rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysates are added followed by a total Lck mouse detection antibody and anti-mouse IgG, HRP-linked antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-Lck (Tyr505) protein.

Background

Lck belongs to the Src-like non-receptor tyrosine kinase family with the typical Src family kinase structure: a unique amino terminal domain (Src homology 4 domain, SH4) followed by an SH3 domain, an SH2 domain, a kinase domain (SH1), and a carboxy-terminal negative regulatory domain (1). Lck activity is controlled by the interactions of SH2 and SH3 domains as well as tyrosine phosphorylation status of the activation loop (2,3). Lck is recruited to the T cell receptor (TCR) complex upon stimulation and activates downstream tyrosine kinases to initiate T cell signaling (4). Lck is also found to be involved in the regulation of mitochondrial apoptosis pathways and may be responsible for some anticancer drug induced apoptosis (5,6).

1. Palacios, E.H. and Weiss, A. (2004) Oncogene 23, 7990-8000.
2. Mustelin, T. and Taskén, K. (2003) Biochem J 371, 15-27.
3. Gervais, F.G. et al. (1993) Mol Cell Biol 13, 7112-21.
4. Straus, D.B. and Weiss, A. (1992) Cell 70, 585-93.
5. Belka, C. et al. (2003) Oncogene 22, 176-85.
6. Gruber, C. et al. (2004) Biochem Pharmacol 67, 1859-72.

注意事项：

Storage: Capture and Detection Antibodies are stored at 4ºC.
Anti-mouse IgG, HRP-linked Antibody is stored at -20ºC.