SignalSilence? ADRM1 siRNA I

• 产品编号：CST-7981S      品牌：CST       原厂货号：7981S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® ADRM1 siRNA I抑制人和猴ADRM1的表达。来自Cell Signaling Technology (CST)的SignalSilence^® ADRM1 siRNA I可以帮助研究者通过RNA干扰特异性地抑制ADRM1的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。CST推荐使用100 nM SignalSilence^® ADRM1 siRNA I进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中siRNA的终浓度为100nM。目前，与蛋白酶体有关的泛素受体有5种：2种蛋白酶体亚基，Rpn10/S5a/PSMD4和Rpn13/ADRM1（黏附-调节分子1），和3种穿梭因子，Rad23, Dsk2, 和Ddi1。ADRM1是一种蛋白酶体的泛素受体(1,2)，其与泛素结合主要经过血小板-白细胞C激酶底物同源结构域，即泛素结构域的血小板-白细胞C激酶底物受体。哺乳动物ADRM1的羧基末端结构域主要结合和加强UCHL5/UCH37异构肽的活性(3-5)，可能可以加速泛素链的分解。ADRM1敲除小鼠的会导致配子发生缺陷，因此表明了哺乳动物发育过程中内源性ADRM1的生理作用(6)。

1. Schreiner, P. et al. (2008) Nature 453, 548-52.
2. Husnjak, K. et al. (2008) Nature 453, 481-8.
3. Yao, T. et al. (2006) Nat Cell Biol 8, 994-1002.
4. Hamazaki, J. et al. (2006) EMBO J 25, 4524-36.
5. Qiu, X.B. et al. (2006) EMBO J 25, 5742-53.
6. Al-Shami, A. et al. (2010) PLoS One 5, e13654.

原厂资料：

Specificity / Sensitivity

SignalSilence^® ADRM1 siRNA I inhibits human and monkey ADRM1 expression.

Description

SignalSilence® ADRM1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit ADRM1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® ADRM1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Currently, there are five ubiquitin receptors associated with the proteasome: two proteasome subunits, Rpn10/S5a/PSMD4 and Rpn13/ADRM1 (Adhesion-regulating molecule 1), and three families of shuttling factors, Rad23, Dsk2, and Ddi1. ADRM1 is a ubiquitin receptor of the proteasome (1,2) that binds ubiquitin via a pleckstrin homology domain known as the pleckstrin-like receptor for ubiquitin (Pru) domain. The carboxy-terminal domain of mammalian ADRM1 serves to bind and enhance the isopeptidase activity of UCHL5/UCH37 (3-5), perhaps serving as a mechanism to accelerate ubiquitin chain disassembly. A murine Adrm1 knockout results in defective gametogenesis, thus highlighting a physiologic role for endogenous ADRM1 in mammalian development (6).

1. Schreiner, P. et al. (2008) Nature 453, 548-52.
2. Husnjak, K. et al. (2008) Nature 453, 481-8.
3. Yao, T. et al. (2006) Nat Cell Biol 8, 994-1002.
4. Hamazaki, J. et al. (2006) EMBO J 25, 4524-36.
5. Qiu, X.B. et al. (2006) EMBO J 25, 5742-53.
6. Al-Shami, A. et al. (2010) PLoS One 5, e13654.

注意事项：

Storage: ADRM1 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC