PathScan? Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair

• 产品编号：CST-7937S      品牌：CST       原厂货号：7937S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体对的特异性和敏感度请参考对应的PathScan® Sandwich ELISA Kit。注意: 此抗体对能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白。CST的PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair是作为PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Kit #7936更为经济的替代而提供的。捕获和检测抗体和HRP连接的二抗分别是作为100X 存储液和1000X存储液而提供。此抗体对为4 x 96孔板的ELISAs提供了足够的试剂。LAT鼠捕获抗体在PBS中加入到96孔板中过夜。封闭后, 加入细胞裂解液然后加入phospho-LAT (Tyr191) 鼠检测抗体和抗兔IgG, HRP连接抗体。 HRP 的底物TMB加入进行显色反应。对反应的颜色，吸收值的增大按比例换算成phospho-LAT (Tyr191)蛋白的量。LAT是跨膜蛋白在T 细胞， NK 细胞和柱状细胞中表达，并在T细胞信号转导中作为重要参与分子(1)。在TCR 招募下, 激活的Zap-70 磷酸化LAT 位于SH2区域的几个保守的酪氨酸残基，使这些区域暴露并作为下游信号的结合位点(2,3)。LAT在 Tyr171 和 Tyr191 位点的磷酸化使得Grb2, Gads/SLP-76, PLCgamma1 和 PI3 激酶通过SH2区域与之结合并在细胞膜上定位。这些过程最终导致了相对应的信号通路的激活(1-4)。

1. Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-6283.
2. Zhang, W. et al. (1998) Cell 92, 83-92.
3. Paz, P. E. et al. (2001) Biochem. J. 356, 461-471.
4. Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-23361.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-LAT (Tyr191) Sandwich ELISA Kit #7936. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The LAT mouse capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a phospho-LAT (Tyr191) rabbit detection antibody and HRP-conjugated, anti-rabbit IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-LAT (Tyr191).

Background

LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling (1). Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets (2,3). The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways (1-4).

1. Wonerow, P. and Watson, S.P. (2001) Oncogene 20, 6273-6283.
2. Zhang, W. et al. (1998) Cell 92, 83-92.
3. Paz, P. E. et al. (2001) Biochem. J. 356, 461-471.
4. Zhang, W. et al. (2000) J. Biol. Chem. 275, 23355-23361.

注意事项：

Storage: LAT-specific mouse capture and phospho-LAT 
(Tyr191)-specific rabbit detection antibodies are stored at 
4ºC. HRP-linked, anti-rabbit IgG antibody is stored at -20ºC.