PathScan? Phospho-Syk (panTyr) Sandwich ELISA Antibody Pair

• 产品编号：CST-7929S      品牌：CST       原厂货号：7929S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体对的特异性和敏感度请参考对应的PathScan® Sandwich ELISA Kit。注意: 此抗体对能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白。CST的PathScan® Phospho-Syk (panTyr) Sandwich ELISA Antibody Pair是作为PathScan® Phospho-Syk (panTyr) Sandwich ELISA Kit #7928更为经济的替代而提供的。捕获和检测抗体和HRP连接的二抗分别是作为100X 存储液和1000X存储液而提供。此抗体对为4 x 96孔板的ELISAs提供了足够的试剂。Syk 鼠捕获抗体在PBS中加入到96孔板中过夜。封闭后, 加入细胞裂解液然后加入生物素化的phospho-tyrosine 鼠检测抗体和HRP连接链霉亲和素。 HRP 的底物TMB加入进行显色反应。对反应的颜色，吸收值的增大按比例换算成酪氨酸磷酸化Syk蛋白的量。Syk作为酪氨酸蛋白激酶在造血细胞的胞内信号转导中发挥重要作用(1-3)。 Syk能够与免疫受体的酪氨酸介导的活性区域(ITAMs)中的胞质部分结合(4)。它能偶联激活了的免疫受体进行下游的信号转导，而引起一系列的细胞应答，如细胞增殖、分化以及吞噬(4)。有证据显示Syk在无免疫反应的细胞中也有作用，Syk在人类的乳腺癌中是个潜在的肿瘤抑制因子(5)。 Syk蛋白的SH2-激酶结合区域中Tyr323位点是个磷酸化负调控位点。磷酸化的Tyr323为 Cbl的TKB区域提供了结合位点(6,7)。Syk的Tyr352位点参与了与 PLC-γ1 的结合 (8)。 Tyr525位点和526位点位于Syk的激酶区域的活性环上。磷酸化人源的Syk的Tyr525/526位点(等同于小鼠 Syk的 Tyr519/520位点) 对 Syk 的功能有重要的影响(9)。

1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-Syk (panTyr) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-Syk (panTyr) Sandwich ELISA Kit #7928. Capture and detection antibodies (100X stocks) and HRP-linked streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Syk mouse capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by biotinylated phospho-tyrosine mouse detection antibody and HRP-linked streptavidin. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Syk phosphorylated on tyrosine's.

Background

Syk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells, and Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation of Tyr323 provides a direct binding site to the TKB domain of Cbl (6,7). Tyrosine 352 of Syk is involved in the association of PLC-γ1 (8). Tyrosines 525 and 526 are located in the activation loop of the Syk kinase domain, and phosphorylation of Tyr525/526 of human Syk (equivalent to the Tyr519/520 of mouse Syk) is essential for Syk function (9).

1. Cheng, A.M. and Chan, A.C. (1997) Curr Opin Immunol 9, 528-33.
2. Kurosaki, T. (1997) Curr Opin Immunol 9, 309-18.
3. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
4. Turner, M. et al. (2000) Immunol Today 21, 148-54.
5. Coopman, P.J. et al. (2000) Nature 406, 742-7.
6. Deckert, M. et al. (1998) J Biol Chem 273, 8867-74.
7. Rao, N. et al. (2001) EMBO J 20, 7085-95.
8. Law, C.L. et al. (1996) Mol Cell Biol 16, 1305-15.
9. Zhang, J. et al. (2000) J Biol Chem 275, 35442-7.

注意事项：

Storage: Capture and detection antibodies are stored at 4ºC.
HRP-linked streptavidin is stored at -20ºC