PathScan? Total IRS-2 Sandwich ELISA Antibody Pair

• 产品编号：CST-7885S      品牌：Cell Signaling Technology       原厂货号：7885S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

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描述：

抗体对的特异性的灵敏性参考相对应的PathScan® Sandwich ELISA Kit。需要注意的是：内部测试证明，该抗体对除了可以检测相应物种的对应蛋白外，也可与其它物种的同源蛋白交互反应。赛信通的PathScan® Total IRS-2 Sandwich ELISA Antibody Pair是PathScan® Total IRS-2 Sandwich ELISA Kit #7884一种更经济实惠的替代产品。试剂盒提供有捕获抗体和检测抗体(100X原液)和HRP结合的链亲和素(1000X原液)。每个试剂盒中的试剂可以满足四个96孔板的ELISA试验。首先在微孔中结合有固相的兔抗IRS-2抗体，然后将细胞裂解液于固相抗体中孵育，IRS-2蛋白被固相抗体所捕获。经过洗脱后，非特异性结合的成分被洗脱下来，然后加入生物素化的兔源IRS-2检测抗体来检测被捕获的IRS-2蛋白，然后再加入辣根过氧化物酶结合的链亲和素来检测特异性结全的检测抗体。最后加入辣根过氧化物酶的底物TMB来进行显色。IRS-2的浓度与显色的强度成正比，因此可以借此对IRS-2定量。胰岛素受体底物2(IRS-2)是胰岛素受体激酶的主要底物之一(1)。在脊椎动物中， IRS-2主要作为一个支架蛋白，用于协调Insulin/IGF信号级联反应中的各个分支通路(2)。IRS-2对于正常的营养稳态是必需的，因为它不仅介导外周组织中的胰岛素功能，还调节着IGF-1对于B细胞生长的作用。IRS-2缺失的小鼠不能维持足够量的胰岛素代偿性分泌，并且容易患1型糖尿病(3)。

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST''s PathScan® Total IRS-2 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total IRS-2 Sandwich ELISA Kit #7884. Capture and detection antibodies (100X stocks) and HRP-conjugated streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 rabbit capture antibody is coated in PBS overnight onto a 96 well microplate. After blocking, cell lysate is added followed by an IRS-2 biotinylated rabbit detection antibody and HRP-linked streptavidin. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

注意事项：

Storage: IRS-2-specific rabbit capture and detection antibodies
are stored at 4ºC. HRP-linked streptavidin is stored at -20ºC.