PathScan? Total GFP Sandwich ELISA Antibody Pair

产品编号：CST-7879S 品牌：CST 原厂货号：7879S
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
应用分类：

包装：

运保温度:

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其它组分：

GFP Capture Ab (100X)【包装：0.4 ml，，运保温度：4°C】

Anti-mouse IgG, HRP-linked Antibody【包装：0.04 ml，，运保温度：–20°C】

GFP Detection Ab (100X)【包装：0.4 ml，，运保温度：4°C】

描述：

对于抗体对的特异性和灵敏度,请参考相应的PathScan® Sandwich ELISA Kit 。注意:这一对蛋白质抗体检测已知物种,已通过内部测试所确定,但是也可以从其他物种中检测同源蛋白质。我公司的这个抗体对是#7878的升级产品。本试剂盒作为检测转染效率的简便方法适用于共转染GFP，可能不能检测GFP融合蛋白。提供捕获和检测抗体（100X stocks）及HRP结合的二抗（1000X stocks）。本产品中的实际可以做4块96孔板的ELISA。在用兔源的抗GFP抗体溶在PBS包被在96孔板每个孔中过夜，封闭后，加入细胞提取物然后加入鼠源的抗GFP抗体检测抗体和HRP直标的抗鼠IgG抗体。加入HRP的底物TMB产生颜色。所产生的颜色的吸光度与GFP的含量呈比例。绿色荧光蛋白（GFP）的功能是作为水母生物发光能量转移受体。GFP可以在395nm最大程度吸收光，其发射光谱高峰在509nm（1）。GFP通常与目的基因共转染来检测转染效率。

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Total GFP Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total GFP Sandwich ELISA Kit #7878. This antibody pair is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency and may not detect GFP fusion proteins. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The GFP rabbit capture antibody is coated onto a 96 well microplate overnight in PBS. After blocking, cell lysate is added followed by a GFP mouse detection antibody and HRP-conjugated, anti-mouse IgG antibody. HRP substrate (TMB) is then added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP.

Background

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency.

注意事项：

Storage: GFP-specific rabbit capture and mouse detection
antibodies are stored at 4ºC. HRP-conjugated, anti-mouse IgG
antibody is stored at -20ºC.