PathScan? Total GFP Sandwich ELISA Kit

产品编号：CST-7878C 品牌：CST 原厂货号：7878C
产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
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其它组分：

ELISA Sample Diluent【包装：25 ml，，运保温度：4°C】

Anti-mouse IgG, HRP-linked Antibody (ELISA Formulated【包装：1 Ea，，运保温度：4°C】

Detection Antibody Diluent【包装：11 ml，，运保温度：4°C】

HRP Diluent【包装：11 ml，，运保温度：4°C】

GFP Mouse Detection mAb【包装：1 Ea，，运保温度：4°C】

STOP Solution 【子货号：#7002，包装：11 ml，，运保温度：4°C】

TMB Substrate 【子货号：#7004，包装：11 ml，，运保温度：4°C】

ELISA Wash Buffer (20X)【包装：25 ml，，运保温度：4°C】

Cell Lysis Buffer (10X) 【子货号：#9803，包装：15 ml，，运保温度：–20°C】

Sealing Tape【包装：2 sheets，，运保温度：4°C】

GFP Ab Coated Microwells【包装：96 tests，，运保温度：4°C】

描述：

该试剂盒可以检测外源表达的GFP，如图1 所示本试剂盒可以在转染后的HEK293细胞提取物中检测到，未转染组则检测不到。本试剂盒作为检测转染效率的简便方法适用于共转染GFP，可能不能检测GFP融合蛋白。这一对蛋白质抗体检测已知物种,已通过内部测试所确定,但是也可以从其他物种中检测同源蛋白质。该试剂盒是一种夹心固相酶联免疫吸附试验（ELISA），可以检测外源表达的GFP。本试剂盒作为检测转染效率的简便方法适用于共转染GFP。用兔源的抗GFP抗体包被每个孔，与细胞提取物一起孵育后，GFP被包被的抗体捕获。经充分洗涤后，鼠源的抗GFP抗体检测抗体加入孔中以检测捕获的GFP。再用HRP直标的抗鼠IgG抗体检测结合的检测抗体。加入HRP的底物TMB产生颜色。所产生的颜色的吸光度与GFP的含量呈比例。绿色荧光蛋白（GFP）的功能是作为水母生物发光能量转移受体。GFP可以在395nm最大程度吸收光，其发射光谱高峰在509nm（1）。GFP的其它用途是通常与目的基因共转染来检测转染效率。

原厂资料：

Specificity / Sensitivity

CST's PathScan® Total GFP Sandwich ELISA Kit #7878 detects GFP when exogenously expressed in cells. As shown in Figure 1, this kit readily detects GFP in transfected, but not in untransfected HEK293 cell lysate. This kit was developed for use with transfected full-length GFP and may not detect GFP fusion proteins. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total GFP Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects exogenously expressed GFP. This kit is intended for use with cotransfected GFP as a convenient means of monitoring transfection efficiency. A GFP rabbit antibody has been coated onto the microwells. After incubation with cell lysates, GFP is captured by the coated antibody. Following extensive washing, a GFP mouse detection antibody is added to detect the captured GFP. Anti-mouse IgG, HRP-linked antibody is then used to recognize the bound detection antibody. The HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of GFP.

Background

The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea. GFP maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm (1). Among other applications, GFP is commonly cotransfected alongside a gene of interest as a means of monitoring transfection efficiency.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).