SignalSilence? PEA-15 siRNA II

• 产品编号：CST-7865S      品牌：CST       原厂货号：7865S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

PEA-15 siRNA II能够抑制人、小鼠、大鼠和猴的Stat6的表达。来自Cell Signaling Technology (CST)的SignalSilence^® PEA-15 siRNA II可以帮助研究者通过RNA干扰特异性地抑制PEA-15的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST 推荐使用100 nM SignalSilence^® PEA-15 siena II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。PEA-15是一个15 kDa的磷酸化蛋白，在星形胶质细胞、成纤维细胞以及组织（包括肺和眼）中表达（1）。已证明该蛋白协助调控细胞的生长、死亡以及葡萄糖利用（2-4）。PEA-15的氨基末端DED域介导它与FADD或Erk的结合，并进一步的调节Erk和细胞凋亡信号通路。PEA-15可以在羧基末端的Ser104和Ser116两个丝氨酸残基处被磷酸化。在这些位点上的磷酸化，可以调节其与Erk和FADD的结合（2,3）。

1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

PEA-15 siRNA II inhibits human, mouse, and rat PEA-15 expression.

Description

SignalSilence^® PEA-15 siRNA II from Cell Signaling Technology (CST) allows the researcher to specifically inhibit PEA-15 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence^® PEA-15 siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose ultilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and futher regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).

1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

注意事项：

Storage: PEA-15 siRNA II is supplied in RNAse-free water.
Aliquot and store at -20ºC.