PathScan? Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair

• 产品编号：CST-7861S      品牌：CST       原厂货号：7861S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

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描述：

抗体对的特异性和灵敏性请参考相对应的PathScan® Sandwich ELISA Kit。内控检测证明，抗体对用于检测相应物种的对应蛋白，但同时也可与其它物种的同源蛋白交互反应。赛信通产品PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair是PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860的更经济实惠的替代型产品。产品包含有捕获抗体和检测抗体(100X原液)，以及抗鼠IgG，辣根过氧化物酶耦联的抗体(1000X原液)。所含试剂可以做四个96孔的ELISA。IRS-2 Rabbit Capture Antibody首先在96孔板中于PBS中孵育过夜，使之紧实地结合到孔中。捕获抗体结合后，加入细胞裂解液，然后再加入Phospho-Tyrosine Mouse Detection Antibody和Anti-Mouse IgG, HRP-linked Antibody。然后加入辣根过氧化物酶的底物TMB使之显色。显色的强度与磷酸化IRS-2 (Tyr)蛋白的浓度成正比。试剂盒中的抗体都是定制的。胰岛素受体底物2(IRS-2)是胰岛素受体激酶的主要底物之一(1)。在脊椎动物中， IRS-2主要作为一个支架蛋白，用于协调Insulin/IGF信号级联反应中的各个分支通路(2)。IRS-2对于正常的营养稳态是必需的，因为它不仅介导外周组织中的胰岛素作用，还调节着IGF-1对于B细胞生长的作用。IRS-2缺失的小鼠不能维持足够量的胰岛素代偿性分泌，并且容易患1型糖尿病(3)。

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST''s PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-IRS-2 (panTyr) Sandwich ELISA Kit #7860. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-2 Rabbit Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-Tyrosine Mouse Detection Antibody and an HRP-conjugated, Anti-Mouse IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-2 phosphorylated on tyrosine. 
 *Antibodies in this kit are custom formulations specific to the kit.

Background

Insulin Receptor Substrate 2 (IRS-2) is one of the major substrates of the insulin receptor kinase (1). In vertebrates, IRS-2 functions as a scaffolding protein to coordinate separate branches of the Insulin/IGF-signaling cascades (2). IRS-2 is essential for normal nutrient homeostasis because it mediates both peripheral insulin action and the effect of IGF-1 on B-cell growth. Mice lacking IRS-2 fail to maintain sufficient compensatory insulin secretion and develop diabetes as young adults (3).

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. White, M.F. (2002) Am. J. Physiol. Endocrinol. Metab. 283, E413-E422.
3. Withers, D.J. et al. (1998) Nature 391, 900-904.

注意事项：

Storage: IRS-2 Rabbit Capture and Phospho-Tyrosine Mouse
Detection Antibodies are stored at 4ºC. Anti-Mouse IgG, HRP-
Linked Antibody is stored at -20ºC.