PathScan? Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair

• 产品编号：CST-7856S      品牌：CST       原厂货号：7856S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

关于该抗体对的特异性和敏感性，请参阅相应的PathScan® Sandwich ELISA试剂盒。注：经过内部测试，该抗体对能够检测指定物种的蛋白，但是也可以检测来自其他物种的同源蛋白。相对于CST的PathScan®Total p21 Waf1/Cip1 Sandwich ELISA Kit #7167，PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair提供了一个经济的选择。我们提供了捕获和检测抗体(100X储备液)和HRP-结合的二级抗体(1000X储备液)，针对4 x 96 孔ELISAs的试剂也非常充分。p21 Waf1/Cip1 mouse capture antibody在含有PBS的96孔板中包被过夜。封闭后加入Aur p21 Waf1/Cip1 rabbit detection antibody和 anti-rabbit IgG ，HRP-conjugated Antibody，随后加入细胞裂解液。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和p21 Waf1/Cip1总蛋白的量成正比。 肿瘤抑制蛋白p21 Waf1/Cip1能够充当细胞周期进程抑制剂。其功能与周期蛋白和周期蛋白依赖性激酶形成的异源三聚体存在化学计量关系。通过与CDK2复合体联合，它可以抑制激酶的活性和阻断G1/S进程(1)。然而，p21也可以增强CDK4或者CDEK6与周期蛋白-D形成的复合体的组装和活性(2)。p21的羧基末端区域能够充分结合并抑制PCNA，DNA聚合酶的一个亚基，而且可以协调细胞周期进程中DNA的复制(3)。基于紫外线损伤或者是在细胞周期中cdc2/cyclin B或CDK2/cyclin A活跃阶段，p53被磷酸化且通过p53-响应元件上调p21的表达(4)。泛素化和蛋白酶体降解能够下调p21的蛋白水平(5)。

1. Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534.
2. Cheng, J. et al. (1999) EMBO J. 18, 1571-1583.
3. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
4. Wang, Y. and Prives, C. (1995) Nature 376, 88-91.
5. Sheaff, R.J. et al. (2000) Cell 5, 403-410.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Total p21 Waf1/Cip1 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan®Total p21 Waf1/Cip1 Sandwich ELISA Kit #7167. Capture and detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The p21 Waf1/Cip1 mouse capture antibody is coated on a 96 well microplate in PBS overnight. After blocking, cell lysates are added followed by a p21 Waf1/Cip1 rabbit detection antibody and anti-rabbit IgG, HRP conjugated antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total p21 Waf1/Cip1 protein.

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A is active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). 

1. Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534.
2. Cheng, J. et al. (1999) EMBO J. 18, 1571-1583.
3. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
4. Wang, Y. and Prives, C. (1995) Nature 376, 88-91.
5. Sheaff, R.J. et al. (2000) Cell 5, 403-410.

注意事项：

Storage: Capture and Detection Antibodies are stored at 4ºC.
Anti-Rabbit IgG, HRP-Linked Antibody is stored at -20ºC.