PathScan? Total Zap-70 Sandwich ELISA Antibody Pair

• 产品编号：CST-7850S      品牌：Cell Signaling Technology       原厂货号：7850S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体对的特异性和敏感度请参考对应的PathScan® Sandwich ELISA Kit。注意: 此抗体对能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白。CST的 PathScan® Total Zap-70 Sandwich ELISA Antibody Pair是作为PathScan® Total Zap-70 Sandwich ELISA Kit #7172更为经济的替代而提供的。捕获和检测抗体和HRP连接的二抗分别是作为100X 存储液和1000X存储液而提供。此抗体对为4 x 96孔板的ELISAs提供了足够的试剂。Zap-70捕获抗体在PBS中加入到96孔板中过夜。封闭后, 加入细胞裂解液然后加入Zap-70检测抗体和抗兔IgG, HRP连接抗体。 HRP 的底物TMB加入进行显色反应。对反应的颜色，吸收值的增大按比例换算成Zap-70蛋白的量。Syk 家族酪氨酸激酶Zap-70 在T 细胞和 NK 细胞中表达并在响应T细胞受体(TCR)而介导T细胞激活中发挥了重要的作用(1)。在TCR 招募下, Zap-70 通过自磷酸化和Src家族酪氨酸激酶Lck转磷酸化作用下快速的磷酸化几个酪氨酸残基(2-6)。酪氨酸的磷酸化与Zap-70激酶活性的增加和下游信号传导有关系。Zap-70 的表达与疾病的进程和慢性淋巴白血病患者的生存率有关(7,8)。

1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Total Zap-70 Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Total Zap-70 Sandwich ELISA Kit #7172. Capture and detection antibodies (100X stocks) and HRP-linked secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Zap-70 capture antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Zap-70 detection antibody and anti-Rabbit IgG, HRP-linked antibody. HRP substrate TMB is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of total Zap-70 protein.

Background

The Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8).

1. Chu, D.H. et al. (1998) Immunol Rev 165, 167-80.
2. Iwashima, M. et al. (1994) Science 263, 1136-9.
3. Neumeister, E.N. et al. (1995) Mol Cell Biol 15, 3171-8.
4. Chan, A.C. et al. (1995) EMBO J 14, 2499-508.
5. Williams, B.L. et al. (1999) EMBO J 18, 1832-44.
6. Di Bartolo, V. et al. (1999) J Biol Chem 274, 6285-94.
7. Wiestner, A. et al. (2003) Blood 101, 4944-51.
8. Crespo, M. et al. (2003) N Engl J Med 348, 1764-75.

注意事项：

Storage: Capture and detection antibodies are stored at 4°C.
Anti-rabbit IgG, HRP-linked antibody is stored at -20°C.