PathScan? Phospho-cdc2 (Tyr15) Sandwich ELISA Antibody Pair

• 产品编号：CST-7838S      品牌：CST       原厂货号：7838S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

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描述：

 抗体对的特异性和敏感性请参照对应的PathScan® Sandwich ELISA Kit。注意：本抗体对能够检测通过内部测试的特定种属蛋白，但也许能够检测其他种属的同源蛋白。 相对于CST的PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit #7176.，PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Antibody Pair提供了一个经济的选择。我们提供了捕获和检测抗体(100X储备液)和HRP-结合的二级抗体(1000X储备液)，针对4 x 96 孔ELISAs的试剂也非常充分。Phospho-cdc2 (Tyr15) Capture Antibody在含有PBS的96孔板中包被过夜。封闭后加入cdc2 Detection Antibody和anti-Mouse IgG, HRP conjugated antibody，随后加入细胞裂解液。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和phospho-cdc2 (Tyr15)蛋白的量成正比。该试剂盒中的抗体具有特异性的固定组成。cdc2蛋白激酶的激活参与调节真核细胞进入有丝分裂，cdc2的激活受多个步骤的调控，包括周期蛋白的结合、cdc2苏氨酸161位的磷酸化(1)。然而，在细胞进入有丝分裂的过程中，激活cdc2的决定性步骤是苏氨酸14位和15位的去磷酸化(2)。Wee1 和 Myt1蛋白激酶通过磷酸化cdc2苏氨酸14位和15位，抑制cdc2活性(3,4)。而cdc25磷酸酯酶可能起到使cdc2苏氨酸14位和15位去磷酸化从而激活cdc2的作用(1,5)。

1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
5. Hunter, T. (1995) Cell 80, 225-36.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. 

Description

CST's PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-cdc2 (Tyr15) Sandwich ELISA Kit #7176. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The Phospho-cdc2 (Tyr15) Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a cdc2 Detection Antibody and anti-Mouse IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-cdc2 (Tyr15) protein. 
 *Antibodies in this kit are custom formulations specific to the kit. 

Background

The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161 (1). However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15 (2). Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases (3,4). The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2 (1,5). 

1. Atherton-Fessler, S. et al. (1994) Mol Biol Cell 5, 989-1001.
2. Norbury, C. et al. (1991) EMBO J 10, 3321-9.
3. McGowan, C.H. and Russell, P. (1993) EMBO J 12, 75-85.
4. Wells, N.J. et al. (1999) J Cell Sci 112 ( Pt 19), 3361-71.
5. Hunter, T. (1995) Cell 80, 225-36.

注意事项：

Storage: Capture and Detection Antibodies are stored at 4ºC.
Anti-Mouse IgG, HRP-Linked Antibody is stored at -20ºC