SignalSilence? hnRNP E1 siRNA I

• 产品编号：CST-7798S      品牌：CST       原厂货号：7798S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Cell Signaling Technology （CST）的SignalSilence^® hnRNP E1 siRNA I 可以通过RNA干扰特异性抑制 hnRNP A1的表达。RNA干扰是一种通过传递双链RNA分子到细胞中从而选择性沉默目的基因表达的方法。所有SignalSilence^® siRNA 产品均经过严格的内部测试并通过Western验证可有效降低目的蛋白的表达。通过三苯基分析对寡核苷酸合成进行逐个碱基的监测以保证适当的耦合效率，随后通过亲和固相提取来纯化寡核苷酸。退火的双链RNA需要通过质谱进行进一步的分析从而确定提取物的组成。为了保证批次间一致性的最大化，每一批次产品都要通过质谱与前一批次的产品进行比较。CST建议使用100 nM SignalSilence ^® hnRNP A1 siRNA I 进行转染，并在48-72小时后裂解细胞。具体的转染操作步骤请根据转染试剂生产商提供的方法进行。如您在使用过程中有任何问题请随时联系我们。hnRNP E1 是参与mRNA前体加工和mRNA转运、定位、稳定性以及翻译的蛋白家族的成员（1-6）。hnRNPE1发挥一系列的生物学功能，如小鼠MOR基因表达的转录激活（7），GHR假外显子表达的选择性剪接的弱化（8），collagen I 和II(9）、beta-球蛋白（10）以及雄激素受体（11）mRNA的稳定性 ，还有包括Dab2、ILEI、和 Bag-1等不同基因的翻译调控（12,13）。hnRNP E1广泛表达。hnRNP E1的磷酸化会影响其与RNA结合的亲和力（13，14）。

1. Cáceres, J.F. et al. (1994) Science 265, 1706-9.
2. Gallouzi, I.E. and Steitz, J.A. (2001) Science 294, 1895-901.
3. Carson, J.H. et al. (2001) Results Probl Cell Differ 34, 69-81.
4. Xu, K. et al. (2001) J Immunol 166, 6358-66.
5. Habelhah, H. et al. (2001) Nat Cell Biol 3, 325-30.
6. Dreyfuss, G. et al. (2002) Nat Rev Mol Cell Biol 3, 195-205.
7. Kim, S.S. et al. (2005) Mol Pharmacol 68, 729-36.
8. Akker, S.A. et al. (2007) Mol Endocrinol 21, 2529-40.
9. Thiele, B.J. et al. (2004) Circ Res 95, 1058-66.
10. Yu, J. and Russell, J.E. (2001) Mol Cell Biol 21, 5879-88.
11. Yeap, B.B. et al. (2002) J Biol Chem 277, 27183-92.
12. Pickering, B.M. et al. (2003) Nucleic Acids Res 31, 639-46.
13. Chaudhury, A. et al. (2010) Nat Cell Biol 12, 286-93.
14. Leffers, H. et al. (1995) Eur J Biochem 230, 447-53.

原厂资料：

Description

SignalSilence^® hnRNP E1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit hnRNP E1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence^® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence^® hnRNP E1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

hnRNP E1 is a member of the hnRNP family of proteins that are involved in pre-mRNA processing and mRNA export, localization, stability, and translation (1-6). hnRNP E1 exerts a wide range of biological functions, such as transcriptional activation of mouse MOR gene expression (7), attenuation of alternative splicing of GHR pseudoexon expression (8), stabilization of collagen I and II (9), beta-globin (10), and androgen receptor (11) mRNAs, and regulation of translation of various genes including Dab2, ILEI, and Bag-1 (12,13). hnRNP E1 is ubiquitously expressed. Phosphorylation of hnRNP E1 affects its RNA binding affinity (13,14).

1. Cáceres, J.F. et al. (1994) Science 265, 1706-9.
2. Gallouzi, I.E. and Steitz, J.A. (2001) Science 294, 1895-901.
3. Carson, J.H. et al. (2001) Results Probl Cell Differ 34, 69-81.
4. Xu, K. et al. (2001) J Immunol 166, 6358-66.
5. Habelhah, H. et al. (2001) Nat Cell Biol 3, 325-30.
6. Dreyfuss, G. et al. (2002) Nat Rev Mol Cell Biol 3, 195-205.
7. Kim, S.S. et al. (2005) Mol Pharmacol 68, 729-36.
8. Akker, S.A. et al. (2007) Mol Endocrinol 21, 2529-40.
9. Thiele, B.J. et al. (2004) Circ Res 95, 1058-66.
10. Yu, J. and Russell, J.E. (2001) Mol Cell Biol 21, 5879-88.
11. Yeap, B.B. et al. (2002) J Biol Chem 277, 27183-92.
12. Pickering, B.M. et al. (2003) Nucleic Acids Res 31, 639-46.
13. Chaudhury, A. et al. (2010) Nat Cell Biol 12, 286-93.
14. Leffers, H. et al. (1995) Eur J Biochem 230, 447-53.

注意事项：

Storage: hnRNP E1 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20°C.