BCA Protein Assay Kit

• 产品编号：CST-7780S      品牌：CST       原厂货号：7780S
• 产品分类：蛋白质检测与分析 > 蛋白标记和检测 > 二级检测试剂盒 > 其他
• 应用分类：

其它组分：

描述：

BCA Protein Assay Kit能用来测量裂解物或匀浆的蛋白质浓度，在微量培养板中的样品，可使用以下缓冲液来准备：Cell Lysis Buffer (10X) #9803，RIPA Buffer (10X) #9806，PathScan® Sandwich ELISA Lysis Buffer (1X) #7018。本方法的检测范围为0.125 - 2 mg/mL。我们建议用BCA来降低还原剂、螯合剂、洗涤剂以及裂解缓冲液中其他常见成分的干扰。具体请参阅操作步骤。二喹啉甲酸(BCA)在碱性环境中能与Cu¹⁺形成一种强紫色的复合物。蛋白质与碱性Cu²⁺反应产生Cu¹⁺。用于常规蛋白质定量方法的基础，该方法能测量宽量程内的蛋白质浓度。随着蛋白质浓度不断增加，产生的颜色越深。BCA protein assay对常规干扰物（如非离子化去污剂和缓冲盐）的耐受性比Lowry technique高（1）。

原厂资料：

Description

The BCA Protein Assay Kit can be used to measure the protein concentration of lysates or homogenates, in microplate format, prepared with the following buffers: Cell Lysis Buffer (10X) #9803, RIPA Buffer (10X) #9806, PathScan® Sandwich ELISA Lysis Buffer (1X) #7018. The dynamic range for this assay is 0.125 - 2 mg/mL. It is recommended that the BCA Compatibility Reagent be used to decrease interference from reducing agents, chelators, detergents, and other common ingredients found in most lysis buffers. Please see the attached protocol for additional details.

Background

Bicinchoninic Acid (BCA) is capable of forming an intense purple complex with cuprous ion, Cu1+, in an alkaline environment. Cu1h+ is produced from the reaction of protein with alkaline Cu2+. The resulting reaction and color produced is the basis for a common protein quantification method capable of measuring protein concentration over a wide range. Increasing protein concentrations produce proportionally deeper colors. The BCA protein assay demonstrates higher tolerances towards common interfering substances, such as nonionic detergents and buffer salts, than the Lowry technique (1).

注意事项：

Storage: This kit is stable at room temperature for 12 months
past the reference date indicated on the kit box label. Do not
freeze and avoid higher than ambient temperatures. Protect from
light: It is recommended to keep reagents within the kit box to
minimize exposure to light.