HMGA1 (D6A4) XP? Rabbit mAb

• 产品编号：CST-7777S      品牌：CST       原厂货号：7777S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HMGA1 (D6A4) XP® Rabbit mAb兔单抗能够识别内源性HMGA1总蛋白、异构体1a和1b。基于序列的同源性，预测该抗体不与HMGA2发生交叉反应。该单克隆抗体是采用合成的与人源HMGA1蛋白Gly68残基周围序列相对应的肽段免疫动物而生产的。HMGA1，原名为HMG-I/Y，属于含有一个AT-hook DNA结合结构域的高迁移率族蛋白家族。HMGA蛋白被认为是架构转录因子；他们不具备直接的转录激活能力，但是相反他们能够通过结合到DNA富含AT的区域来改变DNA构象或者直接与其他转录因子相互作用从而调控基因表达（1,2）。HMGA1在胚胎干细胞以及在胚胎形成期间会高度表达，但不是在完全分化的成年组织中（2-4）。研究发现，HMGA1在快速分裂的肿瘤细胞以及很多侵略性癌症包括甲状腺癌、结肠癌、乳腺癌、胰腺癌以及前列腺癌中一些中会过表达（2-4）。研究者还发现强制表达HMGA1会导致细胞转化和上皮-间充质转变（EMT），而抑制HMGA1表达会阻断非贴壁依赖性细胞的生长以及癌细胞的增殖，这意味着HMGA1通过降低和维持一种去分化的高度增殖的细胞状态来促进肿瘤发生（5-8）。

1. Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
2. Resar, L.M. (2010) Cancer Res 70, 436-9.
3. Chiappetta, G. et al. (1996) Oncogene 13, 2439-46.
4. Ben-Porath, I. et al. (2008) Nat Genet 40, 499-507.
5. Wood, L.J. et al. (2000) Mol Cell Biol 20, 5490-502.
6. Wood, L.J. et al. (2000) Cancer Res 60, 4256-61.
7. Xu, Y. et al. (2004) Cancer Res 64, 3371-5.
8. Scala, S. et al. (2000) Proc Natl Acad Sci U S A 97, 4256-61.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine

Specificity / Sensitivity

HMGA1 (D6A4) XP® Rabbit mAb recognizes endogenous levels of total HMGA1 protein, isoforms 1a and 1b. Based on sequence homology, this antibody is not predicted to cross-react with HMGA2.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly68 of human HMGA1 protein.

Background

HMGA1, formerly known as HMG-I/Y, belongs to a family of high mobility group proteins that contain an AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA1 is highly expressed during embryogenesis and in embryonic stem cells, but not in fully differentiated adult tissues (2-4). Research studies have shown that HMGA1 is over-expressed in rapidly dividing neoplastic cells and a wide variety of aggressive cancers, including thyroid, colon, breast, pancreas, and prostate (2-4). Investigators have shown that forced expression of HMGA1 induces cellular transformation and an epithelial-to-mesenchymal transition (EMT), while inhibition of HMGA1 expression blocks anchorage-independent cell growth and proliferation of cancer cells, suggesting that HMGA1 contributes to carcinogenesis by inducing and maintaining a de-differentiated, highly proliferative cell state (5-8).

1. Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
2. Resar, L.M. (2010) Cancer Res 70, 436-9.
3. Chiappetta, G. et al. (1996) Oncogene 13, 2439-46.
4. Ben-Porath, I. et al. (2008) Nat Genet 40, 499-507.
5. Wood, L.J. et al. (2000) Mol Cell Biol 20, 5490-502.
6. Wood, L.J. et al. (2000) Cancer Res 60, 4256-61.
7. Xu, Y. et al. (2004) Cancer Res 64, 3371-5.
8. Scala, S. et al. (2000) Proc Natl Acad Sci U S A 97, 4256-61.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody