SignalSilence? β2-microglobulin siRNA I

• 产品编号：CST-7759S      品牌：CST       原厂货号：7759S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence® β2-microglobulin siRNA I 能够抑制人源或猴源β2微球蛋白的表达。CST生产的SignalSilence® β2-microglobulin siRNA I可以通过RNA干扰特异性的抑制β2-微球蛋白的表达，此方法是通过传递双链RNA到细胞中从而选择性的沉默目的基因。所有的SignalSilence® siRNA 产品经过严格的公司内部western blot测试并可有效的降低目标蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐转染条件为100 nM SignalSilence® β2-microglobulin siRNA I，48到72小时后裂解细胞。转染步骤需根据转染试剂的方法手册进行。如有问题请联系我们。β2-微球蛋白(B2M)是I类分子组织相容性复合体中重要的成员,是一个三重膜蛋白复合体，其片段来源于结合细胞表面的胞质蛋白，这主要是在免疫系统的监视下识别(1,2)。作为I类组织相容性复合体的成分, β2-微球蛋白在免疫系统的作用中发挥了非常重要的作用(3)。其与癌症的生物学研究有很大的相关性; 例如,研究表明约有三分之一分散型B细胞淋巴瘤中包含了致β2-微球蛋白功能丧失的突变 , 因此就让肿瘤细胞逃脱了免疫系统的监控(4)。此外, β2-微球蛋白被鉴定作为淀粉体前蛋白对胶原质具有亲和性(5);其在长期透析病人的关节炎损伤中积累，成为淀粉体关节炎的致病因素(6)。

1. Krangel, M.S. et al. (1979) Cell 18, 979-91.
2. Collins, E.J. et al. (1995) Proc Natl Acad Sci U S A 92, 1218-21.
3. Marx, J.I. (1974) Science 185, 428-9.
4. Challa-Malladi, M. et al. (2011) Cancer Cell 20, 728-40.
5. Gorevic, P.D. et al. (1985) J Clin Invest 76, 2425-9.
6. Ohashi, K. (2001) Pathol Int 51, 1-10.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Monkey

Specificity / Sensitivity

SignalSilence® β2-microglobulin siRNA I inhibits human, mouse, and monkey β2-microglobulin expression.

Description

SignalSilence® β2-microglobulin siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit β2-microglobulin expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® β2-microglobulin siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

β2-microglobulin (B2M) is a principal component of the Major Histocompatibility Complex (MHC) class I molecule, a ternary membrane protein complex that displays fragments derived from proteolyzed cytosolic proteins on the surface of cells for recognition by the surveillance immune system (1,2). As an integral component of the MHC class I complex, β2-microglobulin plays a critically important role in immune system function (3). It has important relevance to cancer biology research; for example, research studies have shown that nearly one-third of diffuse large B cell lymphomas contain mutations that inactivate β2-microglobulin gene function, thereby allowing tumor cells to escape immune detection (4). In additon, β2-microglobulin has been identified as an amyloid preprotein with collagen-binding affinity (5); its accumulation in osteoarthritic lesions of long-term dialysis patients is reportedly a contributing factor to the condition known as amyloid osteoarthropathy (6).

1. Krangel, M.S. et al. (1979) Cell 18, 979-91.
2. Collins, E.J. et al. (1995) Proc Natl Acad Sci U S A 92, 1218-21.
3. Marx, J.I. (1974) Science 185, 428-9.
4. Challa-Malladi, M. et al. (2011) Cancer Cell 20, 728-40.
5. Gorevic, P.D. et al. (1985) J Clin Invest 76, 2425-9.
6. Ohashi, K. (2001) Pathol Int 51, 1-10.

注意事项：

Storage: Storage: β2-microglobulin siRNA I is supplied in
RNAse-free water. Aliquot and store at -20ºC.