Prestained Protein Marker, Broad Range (Premixed Format)

产品编号：CST-7720S 品牌：Cell Signaling Technology 原厂货号：7720S
产品分类：蛋白质检测与分析 > 蛋白质凝胶电泳 > 蛋白分子量标准品 > 预染蛋白标准品
应用分类：

包装：

70 lanes

运保温度:

–20°C

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描述：

该产品是一种纯化的蛋白与蓝色染料共价结合的混合物，当电泳时分解成7到175KDa之间的8条带（1）。蛋白的浓度与条带的强度经过仔细平衡。在SDS-PAGE中，相对于为预染的蛋白，染料与蛋白的共价结合影响到它们的电泳状态。下表列出了预染蛋白的分子量。（a）将所需要的Prestained Protein Marker转移至另一管中。为了能在电泳中看到条带，请在微型胶中使用15ul，在全长胶中使用30ul。（b）在95-100度，将Marker加热3-5分钟。（c）经快速离心后，直接加到胶的加样孔中。为了保证统一，加入等量的1X reducing SDS Loading Buffer到未用的孔中。 Prestained Protein Marker, Broad Range (Premixed Format) 可在-20度稳定保存至少6个月。

原厂资料：

Description

Prestained Protein Marker, Broad Range is a mixture of purified proteins covalently coupled to a blue dye that resolves to 8 bands between 7 and 175 kDa when electrophoresed (1). The protein concentrations are carefully balanced for even intensity. The covalent coupling of the dye to the proteins affects their electrophoretic behavior in SDS-PAGE gels relative to unstained proteins. The apparent molecular weight of the prestained proteins are given in the table below.

Directions for Use

(a) Transfer the desired amount of the Prestained Protein Marker to a separate tube. For visualizing during electrophoresis: use 15 µl for mini-gels and 30 µl for full length gels.   (b) Heat the Marker to 95-100ºC for 3-5 minutes.   (c) After a quick microcentrifuge spin, load directly onto a gel. To ensure uniform mobility, load an equal volume of 1X reducing SDS Loading Buffer into any unused wells. Prestained Protein Marker, Broad Range (Premixed Format) is stable for a minimum of 6 months at -20ºC.

注意事项：

Storage: Supplied in 70 mM Tris-HCl (pH 6.8 at 25°C), 33 mM NaCl, 1 mM Na2EDTA, 2% (w/v) SDS, 40 mM DTT, 0.01% (w/v) bromophenol blue and 10% glycerol. Store at –20°C.