SignalSilence? CA2 siRNA I

• 产品编号：CST-7670S      品牌：CST       原厂货号：7670S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

Cell Signaling Technology (CST)公司生产的SignalSilence CA2 siRNA I使得研究者可以使用RNA干扰技术特异性的抑制CA2的表达。这种技术通过将双链RNA分子送入细胞而选择性的沉默某个基因的表达。CST的siRNA产品已经在内部经过严格测试，经western分析确能减少目标蛋白的表达。通过三苯分析对寡核苷酸合成进行逐个碱基的监测，确保了正确的耦联效率。然后用亲和固相萃取纯化寡核苷酸。退火的RNA进一步通过质谱分析，以验证其确切成分。每批都将和以前批次进行质谱比对，以确保批次间最大的一致性。CST公司推荐您在细胞裂解前48-72小时用100nM SignalSilence® CA2 siRNA I进行转染。转染的流程请遵循转染试剂制造商提供的操作流程。使用中如有任何问题，请与CST联系。碳酸酐酶（CA）是一个古老的锌金属酶家族成员，在几乎所有活的微生物中都能发现。全部CA可被分为三个亚型（α,β,γ），它们独立进化，在序列和总体折叠上没有明显的同源性。所有功能性CA催化可逆的CO2水合作用为HCO3-和H+，在其活性区域包含一个催化必须的锌原子。哺乳动物CA有很多亚型，它们都属于α亚型（1,2）。

1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.

原厂资料：

Description

SignalSilence® CA2 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit CA2 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® CA2 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Carbonic anhydrases (CA) are a family of ancient zinc metalloenzymes found in almost all living organisms. All CA can be divided into 3 distinct classes (α, β, and γ) that evolved independently and have no significant homology in sequence and overall folding. All functional CA catalyze the reversible hydration of CO2 into HCO3- and H+ and contain a zinc atom in the active sites essential for catalysis. There are many isoforms of CA in mammals and they all belong to the α class (1,2).

1. Smith, K.S. et al. (1999) Proc Natl Acad Sci USA 96, 15184-9.
2. Tripp, B.C. et al. (2001) J Biol Chem 276, 48615-8.

注意事项：

SStorage: CA2 siRNA I is supplied in RNAse-free water. Aliquot
and store at -20ºC.