SignalSilence? hnRNP A1 siRNA I

• 产品编号：CST-7668S      品牌：CST       原厂货号：7668S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® hnRNP A1 siRNA I i能够抑制人、小鼠、大鼠以及猴子中hnRNP D的表达。Cell Signaling Technology （CST）的SignalSilence® hnRNP A1 siRNA I 可以通过RNA干扰特异性抑制 hnRNP A1的表达。RNA干扰是一种通过传递双链RNA分子到细胞中从而选择性沉默目的基因表达的方法。所有SignalSilence^® siRNA 产品均经过严格的内部测试并通过Western验证可有效降低目的蛋白的表达。通过三苯基分析对寡核苷酸合成进行逐个碱基的监测以保证适当的耦合效率，随后通过亲和固相提取来纯化寡核苷酸。退火的双链RNA需要通过质谱进行进一步的分析从而确定提取物的组成。为了保证批次间一致性的最大化，每一批次产品都要通过质谱与前一批次的产品进行比较。CST建议使用100 nM SignalSilence^® hnRNP A1 siRNA I 进行转染，并在48-72小时后裂解细胞。具体的转染操作步骤请根据转染试剂生产商提供的方法进行。如您在使用过程中有任何问题请随时联系我们。Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1)是RNA结合蛋白相关的hnRNP A/B家族成员之一。该类蛋白能够结合pre-mRNA，同时参与RNA的加工、代谢和核内pre-mRNA转录本的转运(1)。hnRNP A1蛋白调控c-Src和c-H-Ras(2,3)的可变剪接，同时影响成纤维细胞生长因子2 mRNA的翻译起始(4)。在许多癌症细胞中hnRNP A1蛋白表达水平上升；并且hnRNP A1基因沉默可导致许多癌细胞的凋亡(5)。虽然hnRNP A1 主要是在细胞核内，但hnRNP A1不断的从细胞核转运到细胞质中，并在这里与mRNA解离并快速被重新转运至核内(6,7)。 hnRNP A1蛋白能结合HIV-1的顺式作用反应序列(CRS)从而影响HIV-1的生成(8,9)。 HIV-1能提高hnRNP A1的表达，并且促进hnRNP A1重新定位到细胞质(10)。

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84.
3. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41.
4. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53.
5. Patry, C. et al. (2003) Cancer Res 63, 7679-88.
6. Piñol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2.
7. Siomi, M.C. et al. (1997) J Cell Biol 138, 1181-92.
8. Black, A.C. et al. (1996) Virus Genes 12, 275-85.
9. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91.
10. Monette, A. et al. (2009) J Biol Chem 284, 31350-62.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

SignalSilence^® hnRNP A1 siRNA I inhibits human, mouse, rat, and monkey hnRNP A1 expression.

Description

SignalSilence^® hnRNP A1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit hnRNP A1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence^® hnRNP A1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

Heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) is a member of the hnRNP A/B family of related RNA binding proteins that bind pre-mRNA and are involved in the processing, metabolism, and transport of nuclear pre-mRNA transcripts (1). hnRNP A1 regulates the alternative splicing of c-Src and c-H-Ras (2,3) and modifies initiation of translation of the fibroblast growth factor 2 mRNA (4). hnRNP A1 expression level is elevated in many cancers; knockdown of hnRNP A1 leads to apoptosis in various cancer cells (5). Although predominantly nuclear, hnRNP A1 is continually transported from the nucleus to the cytoplasm where it disassociates from mRNA and is rapidly re-imported into the nucleus (6,7). hnRNP A1 binds to cis-acting repressive sequences (CRS) of HIV-1 to influence HIV-1 production (8,9). HIV-1 enhances hnRNP A1 expression and promotes the relocalization of hnRNP A1 to the cytoplasm (10).

1. Myer, V.E. and Steitz, J.A. (1995) RNA 1, 171-82.
2. Rooke, N. et al. (2003) Mol Cell Biol 23, 1874-84.
3. Guil, S. et al. (2003) Mol Cell Biol 23, 2927-41.
4. Bonnal, S. et al. (2005) J Biol Chem 280, 4144-53.
5. Patry, C. et al. (2003) Cancer Res 63, 7679-88.
6. Piñol-Roma, S. and Dreyfuss, G. (1992) Nature 355, 730-2.
7. Siomi, M.C. et al. (1997) J Cell Biol 138, 1181-92.
8. Black, A.C. et al. (1996) Virus Genes 12, 275-85.
9. Hadian, K. et al. (2009) J Biol Chem 284, 33384-91.
10. Monette, A. et al. (2009) J Biol Chem 284, 31350-62.

注意事项：

Storage: hnRNP A1 siRNA I is supplied in RNAse-free water.
Aliquot and store at -20ºC.