Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb

• 产品编号：CST-7649L      品牌：CST       原厂货号：7649L
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb 只能检测内源的在Tyr701位点磷酸化的Stat1蛋白。此单克隆抗体是通过合成人源对应的Stat1 Tyr701位点周围的肽段来免疫动物而获得。Stat1转录因子可以被一系列配体所激活(1)， 其中对IFN-α和 IFN-γ的应答非常重要(2,3)。 在 Tyr701位点的磷酸化诱导 Stat1形成二聚体, 入核后与DNA结合(4)。 Stat1 蛋白是以Stat1α (91 kDa) 和 剪接变体Stat1β (84 kDa)二聚体的形式存在。在很多细胞中，这两种形式都是通过IFN-α激活。但是只有 Stat1α是被 IFN-γ激活。Stat1这种不正常的激活方式存在于很多肿瘤(5)。除了酪氨酸磷酸化，Stat1 也能通过p38有丝分裂激活蛋白激酶依赖(MAPK)依赖的通路在Ser727位点发生磷酸化而被激活，从而对IFN-α和其它的细胞应激产生应答(6)。丝氨酸磷酸化对最大化的诱导Stat1介导的基因激活也是必需的。

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-Stat1 (Tyr701) (D4A7) Rabbit mAb recognizes endogenous levels of Stat1 protein only when phosphorylated at Tyr701.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr701 of human Stat1 protein.

Background

The Stat1 transcription factor is activated in response to a large number of ligands (1) and is essential for responsiveness to IFN-α and IFN-γ (2,3). Phosphorylation of Stat1 at Tyr701 induces Stat1 dimerization, nuclear translocation, and DNA binding (4). Stat1 protein exists as a pair of isoforms, Stat1α (91 kDa) and the splice variant Stat1β (84 kDa). In most cells, both isoforms are activated by IFN-α, but only Stat1α is activated by IFN-γ. The inappropriate activation of Stat1 occurs in many tumors (5). In addition to tyrosine phosphorylation, Stat1 is also phosphorylated at Ser727 through a p38 mitogen-activated protein kinase (MAPK)-dependent pathway in response to IFN-α and other cellular stresses (6). Serine phosphorylation may be required for the maximal induction of Stat1-mediated gene activation.

1. Heim, M.H. (1999) J Recept Signal Transduct Res 19, 75-120.
2. Durbin, J.E. et al. (1996) Cell 84, 443-50.
3. Meraz, M.A. et al. (1996) Cell 84, 431-42.
4. Ihle, J.N. et al. (1994) Trends Biochem Sci 19, 222-7.
5. Frank, D.A. (1999) Mol Med 5, 432-56.
6. Wen, Z. et al. (1995) Cell 82, 241-50.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody