Eg5 Antibody

• 产品编号：CST-7625S      品牌：CST       原厂货号：7625S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

该多克隆抗体是由合成的人源的Eg5蛋白羧基末端的肽段免疫动物，采用蛋白A和多肽亲和层析技术纯化生产的。Eg5(也称作驱动蛋白样蛋白 11或者Kif11)属于马达蛋白的驱动蛋白样家族，在染色体定位、中心体分离和有丝分裂纺锤体形成过程中起重要作用。有丝分裂激酶磷酸化Eg5能够通过它与微管的关系调节其活性(1,2)。由于抗有丝分裂化疗药物如紫杉醇，靶向微管并具有多效性，有时甚至是毒性作用，现在靶向微管相关蛋白药物如Eg5正在开发中(3-5)。

1. Blangy, A. et al. (1995) Cell 83, 1159-69.
2. Rapley, J. et al. (2008) J Cell Sci 121, 3912-21.
3. Sarli, V. and Giannis, A. (2006) ChemMedChem 1, 293-8.
4. Cox, C.D. et al. (2008) J Med Chem 51, 4239-52.
5. Nakai, R. et al. (2009) Cancer Res 69, 3901-9.

原厂资料：

Specificity / Sensitivity

Eg5 Antibody recognizes endogenous levels of total Eg5 protein. 

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Eg5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules (1,2). Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development (3-5).

1. Blangy, A. et al. (1995) Cell 83, 1159-69.
2. Rapley, J. et al. (2008) J Cell Sci 121, 3912-21.
3. Sarli, V. and Giannis, A. (2006) ChemMedChem 1, 293-8.
4. Cox, C.D. et al. (2008) J Med Chem 51, 4239-52.
5. Nakai, R. et al. (2009) Cancer Res 69, 3901-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.