SignalSilence? HtrA2/Omi siRNA I

• 产品编号：CST-7574S      品牌：CST       原厂货号：7574S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

ignalSilence® HtrA2/Omi siRNA I抑制人和猴HtrA2/Omi的表达。来自Cell Signaling Technology (CST)的SignalSilence^® HtrA2/Omi siRNA I 可以帮助研究者通过RNA干扰特异性地抑制HtrA2/Omi的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Stat6 siRNA II 进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。蛋白A2（HtrA2）/Omi是一种丝氨酸蛋白酶，与大肠杆菌的HtrA蛋白(DegP) 同源，研究表明其参与细胞凋亡及蛋白错误折叠引起的降解等过程（1）。最初的研究表明HtrA2存在于细胞核（1）或粗面内质网（2）中，后来的研究证明HtrA2存在于线粒体上，在细胞凋亡过程中被释放出来（3-8）。HtrA2产生时是50 kDa的酶原，之后被裂解成36kDa的成熟蛋白，暴露出一个氨基末端基序（AVPS），AVPS类似于IAP抑制子Smac/Diablo（3-8）。与SMAC一样，HtrA2和IAP家族成员之间的相互作用，如XIAP，拮抗caspase活性的抑制，保护细胞免于凋亡（3-8）。有趣的是，在HtrA2基因敲除小鼠模型中，并没有显示出细胞凋亡的减少，而是出现了纹状体中的神经元缺失和帕金森样表型，这表明HtrA2可能具有神经保护功能（9-11）。该活性与HtrA2的蛋白酶活性有关（9）。此外，研究已经在帕金森病中发现了HtrA2基因功能缺失的突变体（12）。

1. Gray, C.W. et al. (2000) Eur. J. Biochem. 267, 5699-5710.
2. Faccio, L. et al. (2000) J. Biol. Chem. 275, 2581-2588.
3. Suzuki, Y. et al. (2001) Mol. Cell 8, 613-621.
4. Hegde, R. et al. (2002) J. Biol. Chem. 277, 432-438.
5. Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
6. van Loo, G. et al. (2002) Cell Death Differ. 9, 20-26.
7. Verhagen, A.M. et al. (2002) J. Biol. Chem. 277, 445-454.
8. Jones, J.M. et al. (2003) Nature 425, 721-727.
9. Vaux, D.L. and Silke, J. (2003) Cell 115, 251-253.
10. Martins, L.M. et al. (2004) Mol. Cell Biol. 24, 9848-9862.
11. Strauss, K.M. et al. (2005) Hum. Mol. Genet. 14, 2099-2111.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

SignalSilence® HtrA2/Omi siRNA I inhibits human and monkey HtrA2/Omi expression.

Description

SignalSilence® HtrA2/Omi siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit HtrA2/Omi expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® HtrA2/Omi siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

Background

High temperature requirement protein A2 (HtrA2)/Omi is a serine protease with homology to the E. coli HtrA protein (DegP) and is thought to be involved in apoptosis and stress-induced degradation of misfolded proteins (1). While HtrA2 was orignally identified to be present in either the nucleus (1) or endoplasmic reticulum (2), subsequent studies have shown that it localizes in mitochondria and is released during apoptosis (3-8). HtrA2 is produced as a 50 kDa zymogen that is cleaved to generate a 36 kDa mature protein that exposes an amino terminal motif (AVPS) resembling that of the IAP inhibitor Smac/Diablo (3-8). Like Smac, interaction between HtrA2 and IAP family members, such as XIAP, antagonizes their inhibition of caspase activity and protection from apoptosis (3-8). Interestingly, HtrA2 knock-out mice did not show signs of reduced apoptosis, but rather had a loss of neurons in the striatum and a Parkinson's-like phenotype, suggesting that HtrA2 might have a neuroprotective function (9-11). This activity is associated with the protease activity of HtrA2 (9). Furthermore, research studies have shown that loss of function mutations in the HtrA2 gene are associated with Parkinson's disease (12).

1. Gray, C.W. et al. (2000) Eur. J. Biochem. 267, 5699-5710.
2. Faccio, L. et al. (2000) J. Biol. Chem. 275, 2581-2588.
3. Suzuki, Y. et al. (2001) Mol. Cell 8, 613-621.
4. Hegde, R. et al. (2002) J. Biol. Chem. 277, 432-438.
5. Martins, L.M. et al. (2002) J. Biol. Chem. 277, 439-444.
6. van Loo, G. et al. (2002) Cell Death Differ. 9, 20-26.
7. Verhagen, A.M. et al. (2002) J. Biol. Chem. 277, 445-454.
8. Jones, J.M. et al. (2003) Nature 425, 721-727.
9. Vaux, D.L. and Silke, J. (2003) Cell 115, 251-253.
10. Martins, L.M. et al. (2004) Mol. Cell Biol. 24, 9848-9862.
11. Strauss, K.M. et al. (2005) Hum. Mol. Genet. 14, 2099-2111.

注意事项：

Storage: HtrA2/Omi siRNA I is supplied in RNAse-free water.
Aliquot and store at -20°C.