CBP (D9B6) Rabbit mAb

• 产品编号：CST-7425S      品牌：CST       原厂货号：7425S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CBP (D9B6) Rabbit mAb兔单抗能够检测内源性CBP总蛋白水平。该抗体也显示与p300蛋白发生交叉反应。通过重组人源CBP蛋白羧基端周围多肽片段去免疫动物从而制备出此单克隆抗体。CBP (CREB-binding protein)和p300蛋白是高度保守的并且具有与转录共激活因子有关联的功能，它是与转录调节因子和信号分子有关联，使用转录分子去整合多种信号转导通路(1,2)。CBP/p300也包含组蛋白乙酰转移酶(HAT)活性，它允许它们使组蛋白或其它蛋白发生乙酰化(2)。通过PKC使p300蛋白在Ser89位点的磷酸化抑制它的转录活性，并且通过AMPK使相同位点的磷酸化干扰了细胞核受体与p300蛋白的联系(3,4)。Akt使p300蛋白Ser1834位点的磷酸化干扰它与C/EBPβ的联系(5)。生长因子可诱导CBP在Ser437位点磷酸化，这对于CBP被招募到转录复合物上时需要的(6)。CaM激酶IV 使CBP蛋白的Ser302位点磷酸化，这在中枢神经系统中对于CBP依赖的转录活性是需要的(7)。CBP/p300蛋白乙酰化的作用是特别有兴趣(2,8)。p300蛋白Lys1499位点的乙酰化已经被证明能提高它的HAT活性，并且影响很多信号传导(9)。

1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
2. Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
3. Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
4. Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
5. Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
6. Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
7. Impey, S. et al. (2002) Neuron 34, 235-244.
8. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
9. Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.

原厂资料：

Specificity / Sensitivity

CBP (D9B6) Rabbit mAb recognizes endogenous levels of total CBP protein. This antibody also shows some cross-reactivity with p300 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human CBP protein.

Background

CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery (1,2). CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins (2). Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors (3,4). Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ (5). Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex (6). CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS (7). The role of acetylation of CBP/p300 is of particular interest (2,8). Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events (9).

1. Goodman, R.H. and Smolik, S. (2000) Genes Dev 14, 1553-77.
2. Chan, H.M. and La Thangue, N.B. (2001) J. Cell Sci. 114, 2363-2373.
3. Yuan, L.W. and Gambee, J.E. (2000) J. Biol. Chem. 275, 40946-40951.
4. Yang, W. et al. (2001) J. Biol. Chem. 276, 38341-38344.
5. Guo, S. et al. (2001) J. Biol. Chem. 276, 8516-8523.
6. Zanger, K. et al. (2001) Mol. Cell 7, 551-558.
7. Impey, S. et al. (2002) Neuron 34, 235-244.
8. Yuan, L.W. and Giordano, A. (2002) Oncogene 21, 2253-2260.
9. Thompson, P.R. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.