MAPKAPK-3 (D54E4) Rabbit mAb

• 产品编号：CST-7421S      品牌：CST       原厂货号：7421S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MAPKAPK-3 (D54E4) Rabbit mAb兔单抗能识别内源性总MAPKAPK-3 蛋白水平。该抗体不能与MAPKAPK-2、MAPKAPK-5蛋白发生交叉反应。该单克隆抗体是通过用与人源MAPKAPK-3蛋白脯氨酸（34位点）周围残基相一致的合成肽段免疫动物后获得的。MAPKAPK-3的脯氨酸富集胺端有一个潜在SH3结合位点，以及一个推测的ATP结合位点、两个MAP激酶磷酸化位点基序和一个推测的细胞核定位信号。它在核苷酸水平与MAPKAPK-2有72%的相似率，在氨基酸水平与MAPKAPK-2有75%的相似率(1)。已经证明MAPKAPK-3能被细胞中的生长诱导因子和应激刺激而活化。体内研究证明Erk, p38 MAP激酶和Jun胺端激酶能磷酸化并活化MAPKAPK-3，暗示了在有丝分裂原和应激应答中该激酶是信号传导的重要结点(2)。有报道称MAPKAPK-3与E47的磷酸化和活性抑制有关，E47是一个“碱性螺旋-环-螺旋” 转录因子，涉及到组织特异性基因表达和细胞分化的调节(3)。通过细胞核转录因子CREB的磷酸化和活化，MAPKAPK-3也许能有助黄体的成熟 (4)。

1. Sithanandam, G. et al. (1996) Mol. Cell Biol. 16, 868-876.
2. Ludwig, S. et al. (1996) Mol. Cell Biol. 16, 6687-6697.
3. Neufeld, B. et al. (2000) J. Biol. Chem. 275, 20239-20242.
4. Maizels, E.T. et al. (2001) Mol. Endocrinol. 15, 716-733.

原厂资料：

Specificity / Sensitivity

MAPKAPK-3 (D54E4) Rabbit mAb recognizes endogenous levels of total MAPKAPK-3 protein. This antibody does not cross-react with MAPKAPK-2 or MAPKAPK-5.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro34 of human MAPKAPK-3 protein.

Background

MAPKAPK-3 has a single potential SH3-binding site in the proline-rich amino terminus, a putative ATP-binding site, 2 MAP kinase phosphorylation site motifs, and a putative nuclear localization signal. It shares 72% nucleotide and 75% amino acid identity with MAPKAPK-2 (1). MAPKAPK-3 has been shown to be activated by growth inducers and stress stimulation of cells. In vitro studies have demonstrated that Erk, p38 MAP kinase, and Jun amino-terminal kinase are able to phosphorylate and activate MAPKAPK-3, which suggested a role for this kinase as an integrative element of signaling in both mitogen and stress responses (2). MAPKAPK-3 was reported to interact with, phosphorylate, and repress the activity of E47, which is a basic helix-loop-helix transcription factor involved in the regulation of tissue-specific gene expression and cell differentiation (3). MAPKAPK-3 may also support luteal maturation through the phosphorylation and activation of the nuclear transcription factor CREB (4).

1. Sithanandam, G. et al. (1996) Mol. Cell Biol. 16, 868-876.
2. Ludwig, S. et al. (1996) Mol. Cell Biol. 16, 6687-6697.
3. Neufeld, B. et al. (2000) J. Biol. Chem. 275, 20239-20242.
4. Maizels, E.T. et al. (2001) Mol. Endocrinol. 15, 716-733.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.