MAPKAPK-5 (D70A10) Rabbit mAb

• 产品编号：CST-7419S      品牌：CST       原厂货号：7419S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MAPKAPK-5 (D70A10) Rabbit mAb兔单抗能识别内源性总MAPKAPK-5 蛋白水平。该抗体不能与MAPKAPK-2、MAPKAPK-3发生交叉反应。该单克隆抗体是采用合成的与人源MAPKAPK-5蛋白Gly367位点周围氨基酸相一致的肽段免疫动物后获得的MAPKAPK-5属于丝裂原活化蛋白激酶(MAPK)活化的蛋白激酶(MK)亚家族，此亚家族还包括MAPKAPK-2/MK2和MK3/3pK。MK亚家族是MAPK通路下游的蛋白激酶亚家族的一部分，包括核醣体S6激酶(RSKs)、有丝分裂原和应激活化激酶(MSKs)和MAPK作用激酶(MNKs)。所有MKs都由MAPK通路激活，并能介导重要的反应过程（比如基因表达、细胞周期进程），同时还涉及炎性反应和癌症(1,2)。MAPKAPK-5能够与p38 MAPK和细胞外调节激酶(Erk)相结合并使自身活化(3,4)。 已经证实MAPKAPK-5能够被Erk3活化，并作为Erk3的伴侣分子(5,6)。虽然过度表达的MAPKAPK-5与MAPKAPK-2有相似的底物，如HSP27、糖原合成酶 ，但是最近的研究表明MAPKAPK-5敲除小鼠中出现独特的底物和功能特性(7)。

1. Roux, P.P. and Blenis, J. (2004) Microbio.l Mol. Biol. Rev. 68, 320-344.
2. Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
3. New, L. et al. (1998) EMBO J. 17, 3372-3384.
4. Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
5. Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
6. Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
7. Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.

原厂资料：

Specificity / Sensitivity

MAPKAPK-5 (D70A10) Rabbit mAb recognizes endogenous levels of total MAPKAPK-5 protein. This antibody does not cross-react with MAPKAPK-2 or MAPKAPK-3.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly367 of human MAPKAPK-5 protein.

Background

MAPKAPK-5 belongs to the mitogen-activated protein kinase (MAPK) activated protein kinases (MK) subfamily that includes MAPKAPK-2/MK2 and MK3/3pK. The MK subfamily is part of a family of protein kinase subfamilies downstream of MAPK pathways and includes ribosomal S6 kinase (RSKs), mitogen and stress activated kinases (MSKs) and MAPK-interacting kinases (MNKs). All MKs are activated by MAPK pathways and mediate important processes (e.g. gene expression, cell cycle progression) and have been implicated in inflammation and cancer (1,2). MAPKAPK-5 shows binding to and activation by p38 MAPK and extracellular-regulated kinases (Erk) (3,4). MAPKAPK-5 was shown to be activated by Erk3 and act as a chaperone to Erk3 (5,6). While overexpressed MAPKAPK-5 shares similar substrates with MAPKAPK-2, such as HSP27 and glycogen synthase, recent work with MAPKAPK-5 knock-out mice indicates distinct substrates and functional properties (7).

1. Roux, P.P. and Blenis, J. (2004) Microbio.l Mol. Biol. Rev. 68, 320-344.
2. Gaestel, M. (2006) Nat. Rev. Mol. Cell Biol. 7, 120-130.
3. New, L. et al. (1998) EMBO J. 17, 3372-3384.
4. Ni, H. et al. (1998) Biochem. Biophys. Res. Commun. 243, 492-496.
5. Schumacher, S. et al. (2004) EMBO J. 23, 4770-4779.
6. Seternes, O.M. et al. (2004) EMBO J. 23, 4780-4791.
7. Shi, Y. et al. (2003) Mol. Cell Biol. 23, 7732-7741.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody