FoxC1 Antibody

• 产品编号：CST-7415S      品牌：Cell Signaling Technology       原厂货号：7415S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

FoxC1可以识别内源性的总FoxC1蛋白。合成与人源FoxC1蛋白的Tyr64氨基酸残基序列一致的肽段，免疫动物获得多克隆抗体。抗体通过蛋白A和肽亲和层析纯化。叉头盒蛋白（Fox）是一组进化中较保守的转录因子，它们拥有一个翼状的螺旋DNA结合结构域而被称为叉头盒（1）。人体内，有可以分成19个亚型的超过40种Fox蛋白，，它们贯穿动物发育的不同方面，调节基因转录（2）。Fox基因突变引起的表达抑制将导致多种人体疾病，包括免疫紊乱，不孕不育，语言功能发育不全和癌症（3,4）。FoxC1 (FKHL7, FREAC3)属于两个哺乳动物FoxC亚家族成员中的一员。与FoxC2一致，它由Wnt/β-catenin调控的轴旁中胚层表达，功能包括促进体节形成，肌形成和脉管发育（5）。FoxC1突变认为是导致双行睫、淋巴水肿综合征的主要原因（6-8），并涉及前节发育不全（ASD），包括先天青光眼和Axenfeld-Rieger 综合征（9）。FoxC1表达水平的变化与乳腺癌侵润相关（10,11）并被证明可以在体外调节乳癌腺癌细胞的增殖和迁移（12）。

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.
5. Savage, J. et al. (2010) Differentiation 79, 31-40.
6. Weisschuh, N. et al. (2008) Clin Genet 74, 476-80.
7. Dejeux, E. et al. (2010) Mol Cancer 9, 68.
8. Muggerud, A.A. et al. (2010) Breast Cancer Res 12, R3.
9. Ray, P.S. et al. (2010) Cancer Res 70, 3870-6.

原厂资料：

Specificity / Sensitivity

FoxC1 Antibody recognizes endogenous levels of total FoxC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr64 of human FoxC1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors defined by the presence of a winged helix DNA binding domain called a Forkhead box (1). In humans, there are over 40 known Fox protein family members, divided into 19 subfamilies, which have evolved to regulate gene transcription in diverse and highly specialized biological contexts throughout animal development (2). Mutations that disrupt the expression of Fox gene family members have consequently been implicated in a broad array of human disorders, including immunological dysfunction, infertility, speech/language disorders, and cancer (3,4).FoxC1 (FKHL7, FREAC3) is one of two mammalian FoxC subfamily members. Along with FoxC2, it is expressed in paraxial mesoderm where it functions to promote somitogenesis, myogenesis, and vascular development, possibly under Wnt/β-catenin regulation (5). Mutations in FoxC1 have been identified as a primary causative factor in lymphedema-distichiasis syndrome (6-8), and are implicated in anterior segment dysgenesis (ASD) disorders, including congenital glaucoma and Axenfeld-Rieger syndrome (9). Alterations in FoxC1 expression have been linked to breast cancer invasiveness (10,11) and have been shown to modulate proliferation and migration of breast cancer cells in vitro (12).

1. Myatt, S.S. and Lam, E.W. (2007) Nat Rev Cancer 7, 847-59.
2. Jackson, B.C. et al. (2010) Hum Genomics 4, 345-52.
3. Hannenhalli, S. and Kaestner, K.H. (2009) Nat Rev Genet 10, 233-40.
4. Benayoun, B.A. et al. (2011) Trends Genet 10, 224-32.
5. Savage, J. et al. (2010) Differentiation 79, 31-40.
6. Weisschuh, N. et al. (2008) Clin Genet 74, 476-80.
7. Dejeux, E. et al. (2010) Mol Cancer 9, 68.
8. Muggerud, A.A. et al. (2010) Breast Cancer Res 12, R3.
9. Ray, P.S. et al. (2010) Cancer Res 70, 3870-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.