PathScan? Total CREB Sandwich ELISA Kit

• 产品编号：CST-7390C      品牌：CST       原厂货号：7390C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

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描述：

PathScan® Total CREB Sandwich ELISA Kit #7390可识别内源性的CREB，如图1所示。试剂盒敏感性如图2所示。本试剂盒可检测经内部测试确定的指定物种的蛋白质，同时也可以检测其他物种的同源蛋白。该试剂盒与CREB相关蛋白ATF-1有交叉反应。PathScan® Total CREB Sandwich ELISA Kit是固相夹心酶联免疫吸附试验（ELISA）试剂盒，识别内源性的CREB。CREB兔抗体已经被包被在微孔上。在和细胞裂解液孵育时，CREB蛋白（磷酸化和非磷酸化）将被包被抗体捕获。在接下来的洗涤后，CREB小鼠检测抗体将结合上，以便识别捕获的CREB蛋白。HRP-偶联的抗鼠IgG将用来识别检测抗体。HRP底物TMB将用来显色。显色后的吸光程度正比于CREB的量。试剂盒中的抗体专为该试剂盒定制。CREB的是一个bZIP转录因子，通过cAMP反应元件激活靶基因。CREB能够介导众多的生理刺激信号，调节很多的细胞反应。CREB的在许多组织中表达，它神经系统起着一个重要的调节作用。CREB被认为在促进特定神经元的存活、增殖、突起生长和神经细胞分化中发挥了关键作用（1-3）。此外，CREB信号在数种生物体中涉及学习和记忆（4-6）。CREB能够通过与不同二聚体相互作用，选择性地激活众多的下游基因。CREB因Ser133的磷酸化而激活，激活可由多种信号通路完成，包括ERK，Ca2+和压力信号。CREB在Ser133的磷酸化所涉及激酶包括p90RSK，MSK，CaMKIV， MAPKAPK-2（7-9）。

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

原厂资料：

Specificity / Sensitivity

PathScan® Total CREB Sandwich ELISA Kit #7390 detects endogenous levels of CREB protein as shown in Figure 1. Kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species. The kit also cross reacts with the CREB-related protein ATF-1.

Description

The PathScan® Total CREB Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of CREB. A CREB rabbit antibody has been coated onto the microwells. After incubation with cell lysates, CREB (phospho and nonphospho) is captured by the coated antibody. Following extensive washing, a CREB mouse detection antibody is added to detect captured CREB protein. Anti-mouse IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total CREB protein. 
 Antibodies in kit are custom formulations specific to kit.

Background

CREB is a bZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth, and neuronal differentiation in certain neuronal populations (1-3). Additionally, CREB signaling is involved in learning and memory in several organisms (4-6). CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+, and stress signaling. Some of the kinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV, and MAPKAPK-2 (7-9).

1. Lonze, B.E. et al. (2002) Neuron 34, 371-85.
2. Lee, M.M. et al. (1999) J Neurosci Res 55, 702-12.
3. Redmond, L. et al. (2002) Neuron 34, 999-1010.
4. Dash, P.K. et al. (1990) Nature 345, 718-21.
5. Yin, J.C. et al. (1994) Cell 79, 49-58.
6. Guzowski, J.F. and McGaugh, J.L. (1997) Proc Natl Acad Sci USA 94, 2693-8.
7. Xing, J. et al. (1998) Mol Cell Biol 18, 1946-55.
8. Ribar, T.J. et al. (2000) J Neurosci 20, RC107.
9. Tan, Y. et al. (1996) EMBO J 15, 4629-42.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).