PathScan? Phospho-p53 (Ser15) Sandwich ELISA Kit

• 产品编号：CST-7365C      品牌：CST       原厂货号：7365C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

其它组分：

描述：

CST的PathScan® Total p53 Sandwich ELISA Kit能够检测内源性丝氨酸(15位) 磷酸化的p53蛋白。使用Sandwich ELISA Kit #7365，能够在紫外处理的HT-29细胞中检测到显著诱导的丝氨酸(15位)磷酸化p53蛋白。然而，由PathScan® Total p53 Sandwich ELISA Kit #7370检测的p53总蛋白（磷酸化和非磷酸化）没有改变(如图1)。CST's PathScan® Phospho-p53 (Ser15) Sandwich ELISA Kit是一种固相酶联免疫吸附试剂盒，能够检测内源性丝氨酸(15位) 磷酸化的p53蛋白的水平。微孔板上已包被有一种p53 (7365-15A5) Rabbit mAb*。细胞裂解液孵育之后，p53蛋白(磷酸化和非磷酸化)被包被抗体捕获。洗涤之后，加入phospho-p53 (16G8) Mouse mAb #9286*检测已捕获的磷酸化p53蛋白。使用HRP-linked anti-mouse antibody #7076*识别联胞检测抗体。加入HRP底物(TMB)进行显色。显色过程中吸光度的强度和磷酸化p53蛋白的量成正比。该试剂盒中的抗体具有特异性的固定组成。p53肿瘤抑制蛋白在细胞响应DNA损伤和其它基因组异常的过程中发挥重要作用。p53的激活能够引起细胞周期捕获和DNA修复或细胞凋亡(1)。离体或者在体情况下，p53可以在多个位点被几个蛋白激酶进行磷酸化(2,3)。DNA损伤能够诱导p53的第15位和20位丝氨酸磷酸化，导致p53和其负调节子-癌蛋白MDM2的相互作用减弱(4)。MDM2通过靶向p53促进其泛素化和蛋白酶体降解抑制其累积。P53的第15位和37位丝氨酸可以被ATM，ATR，和DNA-PK磷酸化(5,6)。P53的磷酸化削弱了MDM2的结合，从而促进了它的激活和累积以响应DNA损伤(4,7)。Chk2和Chk1能够磷酸化p53的第20位丝氨酸，增强其四聚化、稳定性和活性(8,9)。p53的第392位丝氨酸可以发生在体磷酸化(10,11)和CAK介导的离体磷酸化(11)。人类肿瘤中p53的第392位丝氨酸磷酸化增加(12)且有报道认为该过程能够影响生长抑制剂的功能、DNA结合和p53的转录激活(10,13,14)。P53的第6位和9位丝氨酸离体或者在体均可以被CK1δ和CK1ε磷酸化(13,15)。P53的第46位丝氨酸磷酸化能够调节p53诱导细胞凋亡的能力(16)。p300和CBP乙酰转移酶能够介导p53的乙酰化。去乙酰化抑制可以阻止MDM2招募p19 (ARF)介导的HDAC1复合体从而稳定p53。乙酰化似乎对于应激反应中p53蛋白的累积起着正向的作用(17)。DNA损伤后，人类p53的第382位赖氨酸(在小鼠为379位赖氨酸)发生在体乙酰化，促进p53-DNA的结合(18)。P53通过与SIRT1蛋白(一种参与细胞衰老和DNA损伤应激的去乙酰酶)相互作用发生去乙酰化(19)。

1. Levine, A.J. (1997) Cell 88, 323-31.
2. Meek, D.W. (1994) Semin Cancer Biol 5, 203-10.
3. Milczarek, G.J. et al. (1997) Life Sci 60, 1-11.
4. Shieh, S.Y. et al. (1997) Cell 91, 325-34.
5. Chehab, N.H. et al. (1999) Proc Natl Acad Sci U S A 96, 13777-82.
6. Honda, R. et al. (1997) FEBS Lett 420, 25-7.
7. Tibbetts, R.S. et al. (1999) Genes Dev 13, 152-7.
8. Shieh, S.Y. et al. (1999) EMBO J 18, 1815-23.
9. Hirao, A. et al. (2000) Science 287, 1824-7.
10. Hao, M. et al. (1996) J Biol Chem 271, 29380-5.
11. Lu, H. et al. (1997) Mol Cell Biol 17, 5923-34.
12. Ullrich, S.J. et al. (1993) Proc Natl Acad Sci U S A 90, 5954-8.
13. Kohn, K.W. (1999) Mol Biol Cell 10, 2703-34.
14. Lohrum, M. and Scheidtmann, K.H. (1996) Oncogene 13, 2527-39.
15. Knippschild, U. et al. (1997) Oncogene 15, 1727-36.
16. Oda, K. et al. (2000) Cell 102, 849-62.
17. Ito, A. et al. (2001) EMBO J 20, 1331-40.
18. Sakaguchi, K. et al. (1998) Genes Dev 12, 2831-41.
19. Solomon, J.M. et al. (2006) Mol Cell Biol 26, 28-38.

原厂资料：

Specificity / Sensitivity

CST's PathScan® Phospho-p53 (Ser15) Sandwich ELISA Kit detects endogenous levels of Phospho-p53 (Ser15) protein. Using this Sandwich ELISA Kit #7365, a significant induction of phospho-p53 (Ser15) in HT-29 cells treated with UV can be detected. However, the level of total p53 (phospho- and non-phospho), detected by PathScan® Total p53 Sandwich ELISA Kit #7370, remains unchanged (Figure 1).

Description

CST's PathScan® Phospho-p53 (Ser15) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Phospho-p53 (Ser15) protein. A p53 (7365-15A5) Rabbit mAb* has been coated onto the microwells. After incubation with cell lysates, Both nonphospho- and phospho-p53 proteins are captured by the coated antibody. Following extensive washing, a phospho-p53 (16G8) Mouse mAb #9286* is added to detect the captured phospho-p53 protein. HRP-linked anti-mouse antibody #7076* is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of optical density for this developed color is proportional to the quantity of phospho-p53 protein. 
 * Antibodies in this kit are custom formulations specific to the kit.

Background

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis (1). p53 is phosphorylated at multiple sites in vivo and by several different protein kinases in vitro (2,3). DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2 (4). MDM2 inhibits p53 accumulation by targeting it for ubiquitination and proteasomal degradation (5,6). p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage (4,7). Chk2 and Chk1 can phosphorylate p53 at Ser20, enhancing its tetramerization, stability, and activity (8,9). p53 is phosphorylated at Ser392 in vivo (10,11) and by CAK in vitro (11). Phosphorylation of p53 at Ser392 is increased in human tumors (12) and has been reported to influence the growth suppressor function, DNA binding, and transcriptional activation of p53 (10,13,14). p53 is phosphorylated at Ser6 and Ser9 by CK1δ and CK1ε both in vitro and in vivo (13,15). Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis (16). Acetylation of p53 is mediated by p300 and CBP acetyltransferases. Inhibition of deacetylation suppressing MDM2 from recruiting HDAC1 complex by p19 (ARF) stabilizes p53. Acetylation appears to play a positive role in the accumulation of p53 protein in stress response (17). Following DNA damage, human p53 becomes acetylated at Lys382 (Lys379 in mouse) in vivo to enhance p53-DNA binding (18). Deacetylation of p53 occurs through interaction with the SIRT1 protein, a deacetylase that may be involved in cellular aging and the DNA damage response (19). 

1. Levine, A.J. (1997) Cell 88, 323-31.
2. Meek, D.W. (1994) Semin Cancer Biol 5, 203-10.
3. Milczarek, G.J. et al. (1997) Life Sci 60, 1-11.
4. Shieh, S.Y. et al. (1997) Cell 91, 325-34.
5. Chehab, N.H. et al. (1999) Proc Natl Acad Sci U S A 96, 13777-82.
6. Honda, R. et al. (1997) FEBS Lett 420, 25-7.
7. Tibbetts, R.S. et al. (1999) Genes Dev 13, 152-7.
8. Shieh, S.Y. et al. (1999) EMBO J 18, 1815-23.
9. Hirao, A. et al. (2000) Science 287, 1824-7.
10. Hao, M. et al. (1996) J Biol Chem 271, 29380-5.
11. Lu, H. et al. (1997) Mol Cell Biol 17, 5923-34.
12. Ullrich, S.J. et al. (1993) Proc Natl Acad Sci U S A 90, 5954-8.
13. Kohn, K.W. (1999) Mol Biol Cell 10, 2703-34.
14. Lohrum, M. and Scheidtmann, K.H. (1996) Oncogene 13, 2527-39.
15. Knippschild, U. et al. (1997) Oncogene 15, 1727-36.
16. Oda, K. et al. (2000) Cell 102, 849-62.
17. Ito, A. et al. (2001) EMBO J 20, 1331-40.
18. Sakaguchi, K. et al. (1998) Genes Dev 12, 2831-41.
19. Solomon, J.M. et al. (2006) Mol Cell Biol 26, 28-38.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).