PathScan? Phospho-IRS-1 (panTyr) Sandwich ELISA Antibody Pair

• 产品编号：CST-7347S      品牌：CST       原厂货号：7347S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体的特异性和灵敏性请参考相关的PathScan®三明治ELISA试剂盒说明。要注意的是，内部试验证明，该抗体用于检测相应物种的蛋白，但也可能与其它物种的同源蛋白交叉反应。赛信通PathScan® Phospho-IRS-1 (panTyr) Sandwich ELISA Antibody Pair是作为PathScan® Phospho-IRS-1 (panTyr) Sandwich ELISA Kit #7133的一个经济型的替代产品。产品包括捕获 抗体和检测抗体(100X原液)和辣根过氧化物酶连二抗(1000X的原液)。试剂可满足四个96孔板ELISA试验所需。兔源的IRS-2捕获抗体结合在96孔板中，并于PBS中孵育过夜。捕获抗体结合后， 依次加入细胞裂解液，磷酸化酪氨酸残基的鼠检测抗体和抗鼠IgG的酶联抗体。最后加入TMB进行显色反应。显色的吸光度大小与酪氨酸磷酸化的胰岛素受体底物1的数量成正比。试剂盒中抗体 是专门定制的。胰岛素受体底物1(IRS-1)是胰岛素受体激酶的一类主要底物(1)。 IRS-1包含多个酪氨酸磷酸化位点，可以作为含SH2结构域蛋白的停靠位点，这些蛋白调控着胰岛素的代谢和促生长功能(2-4) 　。IRS-1还包含着起过30个潜在的丝氨酸/苏氨酸磷酸化位点，307位丝氨酸可以被JNK(5)和IKK(6)磷酸化，789位的丝氨酸可以被SIK-2（是AMPK家族成员）磷酸化(7)。PKC和mTOR途径可以分 别磷酸化IRS-1的612位丝氨酸和636/639位丝氨酸(8,9)。PKCθ可以介导IRS-2在1101位丝氨酸位的磷酸化，此位点的磷酸化会导致细胞胰岛素信号通路的抑制，因此它可能在某些肥胖模型的 胰岛素抵抗中起着某些作用(10)。

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST''s PathScan® Phospho-IRS-1 (panTyr) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-IRS-1 (panTyr) Sandwich ELISA Kit #7133. Capture and detection antibodies (100X stocks) and an HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IRS-1 Rabbit Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysate is added followed by a Phospho-Tyrosine Mouse Detection Antibody and an HRP-conjugated, Anti-Mouse IgG Antibody. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of IRS-1 phosphorylated on tyrosine. 
 *Antibodies in this kit are custom formulations specific to the kit.

Background

Insulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10).

1. Sun, X.J. et al. (1991) Nature 352, 73-77.
2. Sun, X.J. et al. (1992) J. Biol. Chem. 267, 22662-22672.
3. Myers Jr., M.G. et al. (1993) Endocrinology 132, 1421-1430.
4. Wang, L.M. et al. (1993) Science 261, 1591-1594.
5. Rui, L. et al. (1997) J. Clin. Invest. 107, 181-189.
6. Gao, Z. et al. (2002) J. Biol. Chem. 277, 48115-48121.
7. Horike, N. et al. (2003) J. Biol. Chem. 278, 18440-18447.
8. Ozes, O.N. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 4640-4645.
9. De Fea, K. and Ruth, R.A. (1997) Biochemistry 36, 12939-12947.
10. Li, Y. et al. (2004) J. Biol. Chem. 279, 45304-45307.

注意事项：

Storage: IRS-1 Rabbit Capture and Phospho-Tyrosine
Mouse Detection Antibodies are stored at 4ºC. Anti-Mouse
IgG, HRP-Linked Antibody is stored at -20ºC.