PathScan? Phospho-IκBα (Ser32) Sandwich ELISA Antibody Pair

• 产品编号：CST-7343S      品牌：CST       原厂货号：7343S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体对的特异性和敏感度请参考对应的PathScan® Sandwich ELISA Kit。注意: 此抗体对能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白。CST的PathScan® Phospho-IκB-α (Ser32) Sandwich ELISA Antibody Pair是作为PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Kit #7355更为经济的替代品而提供的。捕获和检测抗体和HRP连接的二抗分别是作为100X 存储液和1000X存储液而提供。此抗体对为4 x 96孔板的ELISAs提供了足够的试剂。IκBα捕获抗体在PBS中加入到96孔板中过夜。封闭后, 加入细胞裂解液然后加入Phospho-IκBα (Ser32)检测抗体和抗兔IgG, HRP连接抗体。 HRP 的底物TMB加入进行颜色反应。对反应的颜色，吸收值的增大按比例换算成Phospho-IκBα (Ser32)蛋白的量。*试剂盒中的抗体是经过客户证实试剂盒特异的。NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。磷酸化IκBα 的Ser32 和 Ser36位点后，蛋白水解酶体介导的IκB降解使得 NF-κB释放激活并入核(3-7)。IκBα的磷酸化导致了Rel依赖的转录能够被很多胞外信号激活，包括炎症因子、生长因子和趋化因子 。能够磷酸化IκB这些活性位点的激酶已经得到了鉴定(8)。

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-IκB-α (Ser32) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-IκBα (Ser32) Sandwich ELISA Kit #7355. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The IκBα Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by a Phospho-IκBα (Ser32) Detection Antibody and anti-Rabbit IgG, HRP conjugated antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-IκBα (Ser32) protein. 
 *Antibodies in this kit are custom formulations specific to the kit.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

注意事项：

Storage: Capture and Detection Antibodies are stored at 4o
C.
Anti-Rabbit IgG, HRP-Linked Antibody is stored at -20o
C.