PathScan? Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit

• 产品编号：CST-7337C      品牌：CST       原厂货号：7337C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

其它组分：

描述：

< p>PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit #7337检测在人和小鼠细胞中内源性S6核糖体蛋白的总蛋白水平。通过内部实验确定，该试剂盒可检测指定的物种，但是可能也会检测其它物种的同源性蛋白。PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit是一个固相夹心酶联免疫吸附法（ELISA），通过化学发光读数其能检测内源性S6核糖体总蛋白水平。化学放光ELISAs相对于传统的发光检测经常有一个宽动态范围和更高的灵敏度。该化学发光ELISA（存在于小体积微孔板中），在使用小体积样本时的信号和敏感性都会增加。一个S6核糖体蛋白鼠源包被在微孔中。在与细胞裂解液一起孵育后，S6核糖体蛋白被包被抗体吸附。充分清洗后，S6核糖体蛋白兔源抗体被加入去检测吸附的S6核糖体蛋白总蛋白。然后Anti-rabbit IgG, HRP-linked Antibody被使用去识别结合的检测抗体。加入化学发光试剂以产生信号。发光的多少（以相对光单位测量s，RLU）与S6核糖体蛋白的总蛋白量成正比例。试剂盒里的抗体是专门针对该试剂盒定制的。生长因子和有丝分裂原有效促进维持细胞生长和增殖的一个方面就是通过上调mRNA翻译(1,2)。生长因子和有丝分裂原能诱导p70 S6激酶的激活和随后S6核糖体蛋白的磷酸化。S6核糖体蛋白的磷酸化与mRNA转录本的翻译增加有关，而这个mRNA转录本是在5端非翻译区含有一个寡嘧啶区(2)。这些特殊的mRNA转录本 (5'TOP) 能够编码涉及细胞周期进程的蛋白质如翻译必须的核糖体蛋白和延伸因子(2,3)。重要的S6核糖体蛋白的磷酸化位点包含数个定位在S6蛋白一个小的羧基末端区域残基(Ser235, Ser236, Ser240, and Ser244) (4,5)。

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

原厂资料：

Specificity / Sensitivity

PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit #7337 detects endogenous levels of total S6 ribosomal protein in human and mouse cells. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species

Description

The PathScan® Total S6 Ribosomal Protein Chemiluminescent Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of total S6 ribosomal protein with a chemiluminescent readout. Chemiluminescence ELISAs often have a wider dynamic range and higher sensitivity than conventional chromogenic detection. This chemiluminescent ELISA, which is offered in low volume microplates, shows increased signal and sensitivity while using smaller sample size. A S6 Ribosomal Protein Mouse mAb has been coated on the microwells. After incubation with cell lysates, the S6 ribosomal protein is captured by the coated antibody. Following extensive washing, S6 Ribosomal Protein Rabbit Antibody is added to detect the captured total S6 ribosomal protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. Chemiluminescent reagent is added for signal development. The magnitude of light emission, measured in relative light units (RLU), is proportional to the quantity of total 
 S6 ribosomal protein. 
 Antibodies in kit are custom formulations specific to kit.

Background

One way that growth factors and mitogens effectively promote sustained cell growth and proliferation is by upregulating mRNA translation (1,2). Growth factors and mitogens induce the activation of p70 S6 kinase and the subsequent phosphorylation of the S6 ribosomal protein. Phosphorylation of S6 ribosomal protein correlates with an increase in translation of mRNA transcripts that contain an oligopyrimidine tract in their 5' untranslated regions (2). These particular mRNA transcripts (5'TOP) encode proteins involved in cell cycle progression as well as ribosomal proteins and elongation factors necessary for translation (2,3). Important S6 ribosomal protein phosphorylation sites include several residues (Ser235, Ser236, Ser240, and Ser244) located within a small, carboxy-terminal region of the S6 protein (4,5).

1. Dufner, A. and Thomas, G. (1999) Exp Cell Res 253, 100-9.
2. Peterson, R.T. and Schreiber, S.L. (1998) Curr Biol 8, R248-50.
3. Jefferies, H.B. et al. (1997) EMBO J 16, 3693-704.
4. Ferrari, S. et al. (1991) J Biol Chem 266, 22770-5.
5. Flotow, H. and Thomas, G. (1992) J Biol Chem 267, 3074-8.

注意事项：

Low volume microplate * 12 8-well modules -each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).