EpCAM (VU1D9) Mouse mAb (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-7319S      品牌：CST       原厂货号：7319S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

EpCAM (VU1D9)Mouse mAb（Alexa Fluor® 594标记）可以检测内源性总的EpCAM蛋白。使用NCI-H69小细胞肺癌细胞免疫动物获得单克隆抗体。Cell Signaling Technology公司抗体偶联Alexa Fluor® 594荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联的EpCAM (VU1D9)小鼠单抗#2929显示同样的物种交叉反应。上皮细胞黏附和激活分子（EpCAM/CD326）是一个介导Ca2+依赖的，大多数上皮细胞基底层亲同种抗原黏附的跨膜糖蛋白。EpCAM在成年人的鳞状上皮细胞中不表达，但是在腺癌和鳞状细胞癌中高表达（1）。作为最初发现的肿瘤相关抗体之一，长期以来EpCAM都作为上皮额肿瘤组织的标记。研究证明EpCAM在癌细胞中高表达（2,3）。

1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

原厂资料：

Specificity / Sensitivity

EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 594 Conjugate) detects endogenous levels of total EpCAM protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EpCAM (VU1D9) Mouse mAb #2929.

Background

Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+-independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Research studies have shown that EpCAM is highly expressed in cancer cells (reviewed in 2,3).

1. Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
2. Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
3. Armstrong, A. and Eck, S.L. (2003) Cancer Biol Ther 2, 320-6.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide
and 2 mg/ml BSA. Store at 4ºC. Protect from light. Do not freeze.