PathScan? Phospho-c-Kit (panTyr) Sandwich ELISA Antibody Pair

• 产品编号：CST-7294S      品牌：CST       原厂货号：7294S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
• 应用分类：

其它组分：

描述：

抗体对的特异性和灵敏度请参考相应的PathScan® Sandwich ELISA Kit。注意：内部检测显示，本试剂盒能检测列举出的物种蛋白，但不排除用于其它物种同源蛋白检测的可能。CST公司的PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Antibody Pair是PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Kit #7231的另一种经济型选择，主要提供捕获和检测抗体（100 X贮存液）以及亲和素偶联的HRP（1000X贮存液），可供4 x 96孔ELISA检测使用。用PBS稀释后的抗c-Kit的小鼠源捕获抗体包被96孔板，封闭后加入细胞裂解液孵育，然后加入生物标记的抗酪氨酸磷酸化的小鼠源检测抗体和亲和素偶联的HRP。最后加入HRP的底物TMB进行显色反应。按照相对光单位，发射光强度与磷酸化的c-Kit (panTyr)蛋白的量成正比。试剂盒中的抗体为试剂盒特别订制配方。c-Kit是受体酪氨酸激酶亚族的成员之一，这个家族包扩PDGF、 CSF-1和 FLT3/flk-2受体 (1,2)。它在造血干细胞、肥大细胞、黑色素细胞和生殖细胞等多种细胞的活化和生长过程中起重要作用(3)。通过和配体干细胞因子 (SCF)相结合，c-Kit发生二聚化/寡聚化并自磷酸化。激活的c-Kit能够招募下游含SH2信号传到成分，包括PLCγ、PI3K激酶的p85亚基、 SHP2和 CrkL，并使其酪氨酸磷酸化 (4)。导致c-Kit激酶功能减弱的分子损伤往往和各种发育异常相关(5)，而促使c-Kit组成型活化的突变则能导致肥大细胞增多症和胃肠道间质肿瘤(6)。Tyr719位于激酶催化插入区域。Tyr719位点磷酸化的c-Kit 的能够在体内或体外与PI3K激酶的p85亚基结合(7)。

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
6. Hirota, S. et al. (1998) Science 279, 577-80.
7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-c-Kit (panTyr) Sandwich ELISA Kit #7231. Capture and Detection Antibodies (100X stocks) and HRP-Conjugated Streptavidin (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The c-Kit Mouse Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Biotinylated Phospho-Tyrosine Mouse Detection Antibody and HRP-conjugated streptavidin. HRP substrate (TMB) is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of phospho-c-Kit (panTyr) protein. 
 Antibodies in kit are custom formulations specific to kit.

Background

c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors (1,2). It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells (3). Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (5), and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (7).

1. Martin, F.H. et al. (1990) Cell 63, 203-11.
2. Yarden, Y. et al. (1987) EMBO J 6, 3341-51.
3. Gommerman, J.L. et al. (1997) J Biol Chem 272, 30519-25.
4. Sattler, M. et al. (1997) J Biol Chem 272, 10248-53.
5. Nocka, K. et al. (1990) EMBO J 9, 1805-13.
6. Hirota, S. et al. (1998) Science 279, 577-80.
7. Blume-Jensen, P. et al. (2000) Nat Genet 24, 157-62.

注意事项：

Storage: c-Kit Mouse Capture Antibody and biotinylated
Phospho-Tyrosine Mouse Detection Antibody are stored at
4ºC.