PathScan? Total Cox2 Sandwich ELISA Kit

• 产品编号：CST-7291C      品牌：CST       原厂货号：7291C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

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描述：

PathScan® Total Cox2 Sandwich ELISA Kit能够检测内源水平的Cox2 蛋白的总体水平。此试剂盒中抗体能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白的可能性。CST PathScan® Total Cox2 Sandwich ELISA Kit是固相夹心酶联免疫吸收剂检测试剂盒(ELISA)，它能够检测内源水平Cox2蛋白。 Cox2鼠抗包裹到微孔的外表面。然后与细胞裂解液孵育，Cox2蛋白能够被抗体捕获。经过严格的冲洗后，Cox2兔检测抗体加入检测捕捉到的Cox2蛋白。抗兔IgG, HRP连接抗体用于识别检测抗体的结合。HRP的底物TMB加入进行颜色反应。对反应的颜色，光密度的数值按比例换算成Cox2蛋白的量。试剂盒中的抗体是经过客户证实试剂盒特异的。环氧合酶1(Cox1) 和环氧合酶2 (Cox2)与人源的同系物有60%的相似度，能够促进前列腺产生花生四烯酸(1,2)。 在大多数的组织中Cox1是组成型表达, Cox2 的表达要通过脂多糖(LPS) 和肽聚糖 (PGN)的诱导(3)。 PGN 激活 Ras, 导致了Raf 在 Ser338位点 和 Erk1/2 在 Tyr204位点的磷酸化。MAP 激酶信号通路的激活引起随后的IKKα/β的激活、IκBα在 Ser32/36位点的磷酸化和 NF-κB 的激活。 最终, 转录调控因子NF-κB的激活诱导了 Cox2基因的表达(4)。LPS 和 PGN 的诱导在临床上表现为关节炎和细菌感染，如炎症引发的发热和败血病性休克 (5), 这些使得 Cox2 成为抗炎症药的一个重要靶标(3)。 Cox1 和 Cox2通过加强 γ-分泌酶活性和β淀粉样蛋白产生，在神经疾病阿尔茨海默疾病中起了重要的作用(6).

1. Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
2. Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
3. O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
4. Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
5. Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
6. Qin, W. et al. (2003) J Biol Chem 278, 50970-7.

原厂资料：

Specificity / Sensitivity

PathScan® Total Cox2 Sandwich ELISA Kit detects endogenous levels of total Cox2 protein. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total Cox2 Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Cox2. A Cox2 Mouse Antibody has been coated onto the microwells. After incubation with cell lysates, Cox2 protein is captured by the coated antibody. Following extensive washing, a Cox2 Rabbit Detection Antibody is added to detect the captured Cox2 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate, TMB, is added to develop color. The magnitude of the absorbance for the developed color is proportional to the quantity of Cox2. 
 Antibodies in kit are custom formulations specific to kit.

Background

Cyclooxygenase1 (Cox1) and cyclooxygenase2 (Cox2), family members with 60% homology in humans, catalyze prostaglandin production from arachidonic acid (1,2). While Cox1 expression is constitutive in most tissues, Cox2 expression is induced by lipopolysaccharide (LPS) and peptidoglycan (PGN) (3). PGN activates Ras, leading to phosphorylation of Raf at Ser338 and Erk1/2 at Tyr204. The activation of MAP kinase signaling results in subsequent activation of IKKα/β, phosphorylation of IκBα at Ser32/36, and NF-κB activation. Finally, activation of the transcription factor NF-κB is responsible for the induction of Cox2 expression (4). LPS and PGN induce the clinical manifestations of arthritis and bacterial infections, such as inflammation, fever, and septic shock (5), making Cox2 a useful target for therapeutic anti-inflammatory drugs (3). Cox1 and Cox2 also play a role in the neuropathology of Alzheimer's disease by potentiating γ-secretase activity and β-amyloid generation (6).

1. Xie, W.L. et al. (1991) Proc Natl Acad Sci USA 88, 2692-6.
2. Vane, J.R. et al. (1998) Annu Rev Pharmacol Toxicol 38, 97-120.
3. O'Neill, G.P. et al. (1994) Mol Pharmacol 45, 245-54.
4. Chen, B.C. et al. (2004) J Biol Chem 279, 20889-97.
5. Wang, Q. et al. (2001) Infect Immun 69, 2270-6.
6. Qin, W. et al. (2003) J Biol Chem 278, 50970-7.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).