PathScan? Phospho-Stat6 (Tyr641) Sandwich ELISA Kit

• 产品编号：CST-7275C      品牌：CST       原厂货号：7275C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

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描述：

PathScan® Total Stat6 Sandwich ELISA Kit能够检测人细胞中内源水平的Stat6 蛋白。如图1所示。此试剂盒的灵敏度在图2中显示。此试剂盒中抗体能够检测已经经内部严格测试的物种的蛋白，但是也不排除检测到其它物种中同源性的蛋白的可能性。CST的PathScan® Phospho-Stat6 (Tyr641) Sandwich ELISA Kit是固相夹心酶联免疫吸收剂检测试剂盒(ELISA)，它能够检测内源水平在 Tyr641位点磷酸化的Stat6蛋白。 Stat6鼠抗包裹到微孔的外表面。然后与细胞裂解液孵育，磷酸化或者非磷酸化的Stat6蛋白能够被抗体捕获。经过严格的冲洗后，Phospho-Stat6 (Tyr641)兔检测抗体加入检测捕捉到的磷酸化的Stat6蛋白。抗兔IgG, HRP连接抗体用于识别检测抗体的结合。HRP的底物TMB加入进行颜色反应。对反应的颜色，光密度的数值按比例换算成在Tyr641位点磷酸化的Stat6蛋白的量。试剂盒中的抗体是经过客户证实试剂盒特异的。受到Janus激酶激活后, Stat6 入核并调控细胞素诱导基因的表达。Stat6 通过在Tyr641位点的磷酸化激活，这对IL-4 和 IL-13的应答是必需的(1-4)。另外， 在B细胞系中Stat6被 IFN-α 激活并与 Stat2和 p48形成具有转录活性的复合体(5,6)。蛋白磷酸酶 2A 也参与到 IL-4介导的 Stat6信号通路中 (7)。

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

原厂资料：

Specificity / Sensitivity

PathScan® Phospho-Stat6 (Tyr641) Sandwich ELISA Kit detects endogenous levels of phospho-Stat6 (Tyr641) in human cells, as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Phospho-Stat6 (Tyr641) Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of Stat6 protein phosphorylated at Tyr641. A Stat6 Mouse mAb has been coated onto the microwells. After incubation with cell lysates, Stat6 (phospho or nonphospho) protein is captured by the coated antibody. Following extensive washing, Phospho-Stat6 (Tyr641) Rabbit Detection Antibody is added to detect the captured phospho-Stat6 protein. Anti-rabbit IgG, HRP-linked Antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of Stat6 phosphorylated at Tyr641. 
 Antibodies in kit are custom formulations specific to kit.

Background

Upon activation by Janus kinases, Stat6 translocates to the nucleus where it regulates cytokine-induced gene expression. Stat6 is activated via phosphorylation at Tyr641 and is required for responsiveness to IL-4 and IL-13 (1-4). In addition, Stat6 is activated by IFN-α in B cells, where it forms transcriptionally active complexes with Stat2 and p48 (5,6). Protein phosphatase 2A is also involved in regulation of IL-4-mediated Stat6 signaling (7).

1. Nelms, K. et al. (1999) Ann. Rev. Immunol. 17, 701-738.
2. Malabarba, M.G. et al. (1996) Biochem. J. 319, 865-872.
3. Hou, J. et al. (1994) Science 265, 1701-1706.
4. Quelle, F.W. et al. (1995) Mol. Cell. Biol. 15, 3336-3343.
5. Takeda, K. et al. (1996) Nature 380, 627-630.
6. Gupta, S. et al. (1999) J. Immunol. 163, 3834-3841.
7. Woetmann, A. et al. (2003) J. Biol. Chem. 278, 2787-2791.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).