PathScan? Total GSK-3β Sandwich ELISA Kit

• 产品编号：CST-7265C      品牌：CST       原厂货号：7265C
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测试剂盒
• 应用分类：

其它组分：

描述：

PathScan® Total GSK-3β Sandwich ELISA Kit #7265能够检测内源性水平的总人GSK-3β，见图1.该试剂盒的灵敏度见图2.本试剂盒用来检测指示物种的蛋白，即通过内部测试确定的那些，也可以用来检测来自其他物种的同源蛋白。PathScan® Total GSK-3β Sandwich ELISA Kit是固相夹心酶联免疫吸附试验（ELISA），能够检测内源性水平的GSK-3β蛋白。微孔包被GSK-3β鼠单克隆抗体。细胞裂解液孵育后，GSK-3β（磷酸和非磷酸）蛋白由包被的抗体捕获。充分冲洗后，加入GSK-3β兔单克隆抗体检测捕获的GSK-3β蛋白。随后用抗兔IgG，HRP-标记的抗体识别结合的检测蛋白。加入HRP底物TMB进行显色。显色的吸光度值与总GSK-3β蛋白的量成正比。试剂盒中的抗体是针对试剂盒定制的。GSK-3是一个广泛表达的丝氨酸/苏氨酸蛋白激酶，磷酸化并使糖原合成酶失活。GSK-3是PI3K/Akt细胞存活通路的一个关键下调因子，该通路的活性可以被Akt介导的GSK-3α丝氨酸21位和GSK-3β丝氨酸9位的磷酸化抑制（2,3）。GSK-3与粘菌细胞命运的调控有关，是爪蟾、果蝇和哺乳动物发育中Wnt信号通路的一个组成部分（4）。已证明GSK-3能调节细胞周期蛋白D1的蛋白水解和亚细胞定位（5）。

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

原厂资料：

Specificity / Sensitivity

PathScan® Total GSK-3β Sandwich ELISA Kit #7265 detects endogenous levels of total human GSK-3β as shown in Figure 1. The kit sensitivity is shown in Figure 2. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

The PathScan® Total GSK-3β Sandwich ELISA Kit is a solid phase sandwich enzyme-linked immunosorbent assay (ELISA) that detects endogenous levels of GSK-3β protein. A GSK-3β mouse mAb has been coated onto the microwells. After incubation with cell lysates, GSK-3β (phospho and nonphospho) protein is captured by the coated antibody. Following extensive washing, a GSK-3β rabbit mAb is added to detect the captured GSK-3β protein. Anti-rabbit IgG, HRP-linked antibody is then used to recognize the bound detection antibody. HRP substrate TMB is added to develop color. The magnitude of the absorbance for this developed color is proportional to the quantity of total GSK-3β. 
 Antibodies in kit are custom formulations specific to kit.

Background

Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).

1. Welsh, G.I. et al. (1996) Trends Cell Biol 6, 274-9.
2. Srivastava, A.K. and Pandey, S.K. (1998) Mol Cell Biochem 182, 135-41.
3. Cross, D.A. et al. (1995) Nature 378, 785-9.
4. Nusse, R. (1997) Cell 89, 321-3.
5. Diehl, J.A. et al. (1998) Genes Dev 12, 3499-511.

注意事项：

* 12 8-well modules -Each module is designed to break apart for 8 tests.
**Kit should be stored at 4°C with the exception of 10X Cell Lysis Buffer, which is stored at –20°C (packaged separately).