PathScan? Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Antibody Pair

• 产品编号：CST-7217S      品牌：CST       原厂货号：7217S
• 产品分类：ELISA及其它免疫分析 > ELISA > ELISA检测抗体对
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描述：

对于Antibody Pair的特异性和灵敏度，请参照相关的PathScan® Sandwich ELISA Kit。注意：经内部测试确认，本抗体对可以检测到所列物种中的蛋白。但对其他物种中的同源蛋白可能也可以检测。CST的 PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Antibody Pair是可以替代PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Kit #7325的更经济的产品。其中提供了捕获抗体、检测抗体(100X stocks)和HRP偶联的二抗(1000X stock)。这些试剂足够完成4 x 96 孔板的ELISAs实验。在96孔板中，加入SAPK/JNK捕获抗体进行包被。封闭后，加入细胞裂解液，随后分别加入Phospho-SAPK/JNK (Thr183/Tyr185) 检测抗体和anti-Rabbit IgG的HRP偶联抗体。最后加入HRP底物—— TMB，进行显色。产生的吸光值与Phospho-SAPK/JNK (Thr183/Tyr185) 蛋白的数量成正比。*试剂盒中抗体是专为本试剂盒特制的。应激活化蛋白激酶/Jun氨基末端激酶SAPK/JNK能够有效、优先地被多种环境压力激活，这些环境压力包括紫外照射和gamma 辐射、神经酰胺、炎性因子，在某些情况下也包括生长因子和GPCR激动剂 (1-6)。正如其他的 MAPKs，核心信号传导单元包括一个MAPKKK，通常是MEKK1-MEKK4，或一种混合谱系酶 (MLKs），这些MAPKKK激酶能磷酸化并激活 MKK4/7。活化后，MKKs磷酸化并激活SAPK/JNK 激酶 (2)。应激反应信号经Rho 家族(Rac, Rho, cdc42)的小GTP酶被传递到这个级联信号反应中 (3)。Rac1 和 cdc42蛋白都能调节MEKKs、 MLKs的激活(3)。另外一种情况是，MKK4/7能够通过生发中心激酶 (GCK)家族成员的刺激，在一个非GTP酶依赖机制中被激活 (4)。有三种不同的SAPK/JNK基因，而每一种都会经过选择性剪切产生多种异构体(3)。活化的二聚体SAPK/JNK，能转位到细胞核中，通过它对 c-Jun, ATF-2和其他转录因子的效应而调节转录过程(3,5)。

1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
2. Ichijo, H. (1999) Oncogene 18, 6087-93.
3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

原厂资料：

Specificity / Sensitivity

For Antibody Pair specificity and sensitivity, please refer to the corresponding PathScan® Sandwich ELISA Kit. Note: This antibody pair detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.

Description

CST's PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Antibody Pair is being offered as an economical alternative to our PathScan® Phospho-SAPK/JNK (Thr183/Tyr185) Sandwich ELISA Kit #7325. Capture and Detection antibodies (100X stocks) and HRP-conjugated secondary antibody (1000X stock) are supplied. Sufficient reagents are supplied for 4 x 96 well ELISAs. The SAPK/JNK Capture Antibody is coated in PBS overnight in a 96 well microplate. After blocking, cell lysates are added followed by Phospho-SAPK/JNK (Thr183/Tyr185) Detection Antibody and Anti-rabbit IgG, HRP-conjugated Antibody. HRP substrate, TMB, is added for color development. The magnitude of the absorbance for this developed color is proportional to the quantity of Phospho-SAPK/JNK (Thr183/Tyr185) protein. 
 *Antibodies in this kit are custom formulations specific to the kit.

Background

The stress-activated protein kinase/Jun-amino-terminal kinase SAPK/JNK is potently and preferentially activated by a variety of environmental stresses including UV and gamma radiation, ceramides, inflammatory cytokines, and in some instances, by growth factors and GPCR agonists (1-6). As with the other MAPKs, the core signaling unit is composed of a MAPKKK, typically MEKK1-MEKK4, or by one of the mixed lineage kinases (MLKs), which phosphorylate and activate MKK4/7. Upon activation, MKKs phosphorylate and activate the SAPK/JNK kinase (2). Stress signals are delivered to this cascade by small GTPases of the Rho family (Rac, Rho, cdc42) (3). Both Rac1 and cdc42 mediate the stimulation of MEKKs and MLKs (3). Alternatively, MKK4/7 can be activated in a GTPase-independent mechanism via stimulation of a germinal center kinase (GCK) family member (4). There are three SAPK/JNK genes each of which undergoes alternative splicing resulting in numerous isoforms (3). SAPK/JNK, when active as a dimer, can translocate to the nucleus and regulate transcription through its effects on c-Jun, ATF-2, and other transcription factors (3,5).

1. Davis, R.J. (1999) Biochem Soc Symp 64, 1-12.
2. Ichijo, H. (1999) Oncogene 18, 6087-93.
3. Kyriakis, J.M. and Avruch, J. (2001) Physiol Rev 81, 807-69.
4. Kyriakis, J.M. (1999) J Biol Chem 274, 5259-62.
5. Leppä, S. and Bohmann, D. (1999) Oncogene 18, 6158-62.
6. Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem Sci 23, 481-5.

注意事项：

Storage: SAPK/JNK Capture and Phospho-SAPK/JNK
(Thr183/Tyr185) Detection Antibodies are stored at 4 ° C. Anti-
rabbit IgG, HRP-Linked Antibody is stored at -20 ° C.